This study aims to elucidate the antiproliferative mechanism of hydroxychavicol(HC).Its effects on cell cycle,apoptosis,and the expression of c-Jun N-terminal kinase(JNK)and P38 mitogen-activated protein kinase(MAPK)i...This study aims to elucidate the antiproliferative mechanism of hydroxychavicol(HC).Its effects on cell cycle,apoptosis,and the expression of c-Jun N-terminal kinase(JNK)and P38 mitogen-activated protein kinase(MAPK)in HT-29 colon cancer cells were investigated.HC was isolated from Piper betle leaf(PBL)and verified by high-performance liquid chromatography(HPLC),nuclear magnetic resonance(NMR),and gas chromatography-mass spectrometry(GC-MS).The cytotoxic effects of the standard drug 5-fluorouracil(5-FU),PBL water extract,and HC on HT-29 cells were measured after 24,48,and 72 h of treatment.Cell cycle and apoptosis modulation by 5-FU and HC treatments were investigated up to 30 h.Changes in phosphorylated JNK(pJNK)and P38(pP38)MAPK expression were observed up to 18 h.The half maximal inhibitory concentration(IC_(50))values of HC(30μg/mL)and PBL water extract(380μg/mL)were achieved at 24 h,whereas the IC_(50)of 5-FU(50μmol/L)was obtained at 72 h.Cell cycle arrest at the G0/G1 phase in HC-treated cells was observed from12 h onwards.Higher apoptotic cell death in HC-treated cells compared to 5-FU-treated cells(P<0.05)was observed.High expression of pJNK and pP38 MAPK was observed at 12 h in HC-treated cells,but not in 5-FU-treated HT-29 cells(P<0.05).It is concluded that HC induces cell cycle arrest and apoptosis of HT-29 cells,with these actions possibly mediated by JNK and P38 MAPK.展开更多
Piper betle(PB),also known as ubeter,in Malay language,is a tropical Asian vine.PB leaves are commonly chewed by Asians along with betel quid.It contains phenols such as eugenol and hydroxychavicol along with chloroph...Piper betle(PB),also known as ubeter,in Malay language,is a tropical Asian vine.PB leaves are commonly chewed by Asians along with betel quid.It contains phenols such as eugenol and hydroxychavicol along with chlorophyll,P-carotene,and vitamin C(Salehi et al.,2019).Extracts from PB leaves have various medicinal properties including anticancer,antioxidant,anti-inflammatory,and antibacterial effects(Salehi et al.,2019).Previous research has shown that PB induces cell cycle arrest at late S or G2/M phase and causes apoptosis at higher doses(Wu et al.,2014;Guha Majumdar and Subramanian,2019).展开更多
基金supported by the Taylor’s Research Grant Scheme(No.TRGS/MFS/2/2013/SBS/003),Malaysia。
文摘This study aims to elucidate the antiproliferative mechanism of hydroxychavicol(HC).Its effects on cell cycle,apoptosis,and the expression of c-Jun N-terminal kinase(JNK)and P38 mitogen-activated protein kinase(MAPK)in HT-29 colon cancer cells were investigated.HC was isolated from Piper betle leaf(PBL)and verified by high-performance liquid chromatography(HPLC),nuclear magnetic resonance(NMR),and gas chromatography-mass spectrometry(GC-MS).The cytotoxic effects of the standard drug 5-fluorouracil(5-FU),PBL water extract,and HC on HT-29 cells were measured after 24,48,and 72 h of treatment.Cell cycle and apoptosis modulation by 5-FU and HC treatments were investigated up to 30 h.Changes in phosphorylated JNK(pJNK)and P38(pP38)MAPK expression were observed up to 18 h.The half maximal inhibitory concentration(IC_(50))values of HC(30μg/mL)and PBL water extract(380μg/mL)were achieved at 24 h,whereas the IC_(50)of 5-FU(50μmol/L)was obtained at 72 h.Cell cycle arrest at the G0/G1 phase in HC-treated cells was observed from12 h onwards.Higher apoptotic cell death in HC-treated cells compared to 5-FU-treated cells(P<0.05)was observed.High expression of pJNK and pP38 MAPK was observed at 12 h in HC-treated cells,but not in 5-FU-treated HT-29 cells(P<0.05).It is concluded that HC induces cell cycle arrest and apoptosis of HT-29 cells,with these actions possibly mediated by JNK and P38 MAPK.
基金Project supported by the Taylor’s Research Grant Scheme(No.TRGS/MFS/2/2013/SBS/003)。
文摘Piper betle(PB),also known as ubeter,in Malay language,is a tropical Asian vine.PB leaves are commonly chewed by Asians along with betel quid.It contains phenols such as eugenol and hydroxychavicol along with chlorophyll,P-carotene,and vitamin C(Salehi et al.,2019).Extracts from PB leaves have various medicinal properties including anticancer,antioxidant,anti-inflammatory,and antibacterial effects(Salehi et al.,2019).Previous research has shown that PB induces cell cycle arrest at late S or G2/M phase and causes apoptosis at higher doses(Wu et al.,2014;Guha Majumdar and Subramanian,2019).
