Based on the unique advantages of fluorescent silicon nanoparticles （SiNPs）, long circulation red blood cells （RBCs）, and anti-cancer drug molecules （i.e., doxorubicin （DOX））, we developed multifunctional DOX-...Based on the unique advantages of fluorescent silicon nanoparticles （SiNPs）, long circulation red blood cells （RBCs）, and anti-cancer drug molecules （i.e., doxorubicin （DOX））, we developed multifunctional DOX-loaded SiNPs impregnated into RBCs. Importantly, the resulting drug delivery systems （DDSs） simultaneously exhibited bright fluorescence coupled with robust photostability （i.e., - 24% loss of fluorescent intensity after 25 min continuous laser irradiation） and significantly lengthened blood residency （i.e., t1/2 = 7.31 ± 0.96 h, 3.9-fold longer than pure DOX-loaded SiNPs）. Therefore, this novel DDS featuring multi-functionalities shows high potential for cancer diagnosis and therapy, particularly for tumor imaging and chemotherapy in a synchronous manner.展开更多
Recommended as a medical emergency,infectious keratitis with an acute and rapid disease progression can lead to serious damage of vision and even blindness.Herein,we present a kind of theranostic agents,which are made...Recommended as a medical emergency,infectious keratitis with an acute and rapid disease progression can lead to serious damage of vision and even blindness.Herein,we present a kind of theranostic agents,which are made of vancomycin(Van)-modified fluorescent silicon nanoparticles(SiNPs-Van),enabling rapid and non-invasive diagnosis and treatment of Gram-positive bacteria-induced keratitis in a simultaneous manner.Typically,the resultant SiNPs-Van nanoagents have an ability of imaging bacteria in a short time both in vitro(5 min)and in vivo(10 min),making them an efficacious diagnostic agent for the detection of bacterial keratitis.In addition,the SiNPs-Van feature distinct antimicrobial activity,with superior activity of 92.5%at a concentration of 0.5 ng/mL against Staphylococcus aureus(S.aureus);comparatively,the antimicrobial rate of free vancomycin is 23.3%at the same concentration.We further explore the SiNPs-Van agents as eye drops for therapy of S.aureus-induced bacterial keratitis on rat model.Represented by slit-lamp scores,the keratitis severity of SiNPs-Van-treated corneas is 3.4,which is 59.6%and 77.3%slighter than vancomycin-(8.2 score)and PBS-treated corneas(15.0 score),respectively.The infected corneas recover to normal(1 score)after 7-d of SiNPs-Van treatment.Above results suggest that the SiNPs-Van could serve as a new kind of high-quality nanotheranostic agents,especially suitable for simultaneous diagnosis and therapy of Gram-positive bacteria keratitis.展开更多
Despite sufficient studies performed in non-primate animal models,there exists scanty information obtained from pilot trials in non-human primate animal models,severely hindering nanomaterials moving from basic resear...Despite sufficient studies performed in non-primate animal models,there exists scanty information obtained from pilot trials in non-human primate animal models,severely hindering nanomaterials moving from basic research into clinical practice.We herein present a pioneering demonstration of nanomaterials based optical imaging-guided surgical operation by using macaques as a typical kind of non-human primate-animal models.Typically,taking advantages of strong and stable fluorescence of the small-sized(diameter:~5 nm)silicon-based nanoparticles(SiNPs),lymphatic drainage patterns can be vividly visualized in a real-time manner,and lymph nodes(LN)are able to be sensitively detected and precisely excised from small animal models(e.g.,rats and rabbits)to non-human primate animal models(e.g.,cynomolgus macaque(Macaca fascicularis)and rhesus macaque(Macaca mulatta)).Compared to clinically used invisible near-infrared(NIR)lymphatic tracers(i.e.,indocyanine green(ICG);etc.),we fully indicate that the SiNPs feature unique advantages for naked-eye visible fluorescence-guided surgical operation in long-term manners.Thorough toxicological analysis in macaque models further provides confirming evidence of favorable biocompatibility of the SiNPs probes.We expect that our findings would facilitate the translation of nanomaterials from the laboratory to the clinic,especially in the field of cancer treatment.展开更多
基金We express our grateful thanks to Prof. Shuit-Tong Lee for general help and valuable suggestion. We appreciate financial support from the National Basic Research Program of China （No. 2013CB934400）, the National Natural Science Foundation of China （Nos. 61361160412 and 31400860）, and a project funded by the Priority Academic Program Development of Jiangsu Higher Education Institutions （PAPD）, 111 Project as well as Collaborative Innovation Center of Suzhou Nano Science and Technology （NANO-CIC）.
文摘Based on the unique advantages of fluorescent silicon nanoparticles （SiNPs）, long circulation red blood cells （RBCs）, and anti-cancer drug molecules （i.e., doxorubicin （DOX））, we developed multifunctional DOX-loaded SiNPs impregnated into RBCs. Importantly, the resulting drug delivery systems （DDSs） simultaneously exhibited bright fluorescence coupled with robust photostability （i.e., - 24% loss of fluorescent intensity after 25 min continuous laser irradiation） and significantly lengthened blood residency （i.e., t1/2 = 7.31 ± 0.96 h, 3.9-fold longer than pure DOX-loaded SiNPs）. Therefore, this novel DDS featuring multi-functionalities shows high potential for cancer diagnosis and therapy, particularly for tumor imaging and chemotherapy in a synchronous manner.
基金the National Natural Science Foundation of China(Nos.21825402,21575096,31400860,and 21605109)Natural Science Foundation of Jiangsu Province of China(Nos.BK20170061,BK20191417)+1 种基金the Program for Jiangsu Specially-Appointed Professors to Prof.Yao He,a project funded by the Priority Academic Program Development of Jiangsu Higher Education Institutions(PAPD)111 Project as well as Collaborative Innovation Center of Suzhou Nano Science and Technology(NANO-CIC).
文摘Recommended as a medical emergency,infectious keratitis with an acute and rapid disease progression can lead to serious damage of vision and even blindness.Herein,we present a kind of theranostic agents,which are made of vancomycin(Van)-modified fluorescent silicon nanoparticles(SiNPs-Van),enabling rapid and non-invasive diagnosis and treatment of Gram-positive bacteria-induced keratitis in a simultaneous manner.Typically,the resultant SiNPs-Van nanoagents have an ability of imaging bacteria in a short time both in vitro(5 min)and in vivo(10 min),making them an efficacious diagnostic agent for the detection of bacterial keratitis.In addition,the SiNPs-Van feature distinct antimicrobial activity,with superior activity of 92.5%at a concentration of 0.5 ng/mL against Staphylococcus aureus(S.aureus);comparatively,the antimicrobial rate of free vancomycin is 23.3%at the same concentration.We further explore the SiNPs-Van agents as eye drops for therapy of S.aureus-induced bacterial keratitis on rat model.Represented by slit-lamp scores,the keratitis severity of SiNPs-Van-treated corneas is 3.4,which is 59.6%and 77.3%slighter than vancomycin-(8.2 score)and PBS-treated corneas(15.0 score),respectively.The infected corneas recover to normal(1 score)after 7-d of SiNPs-Van treatment.Above results suggest that the SiNPs-Van could serve as a new kind of high-quality nanotheranostic agents,especially suitable for simultaneous diagnosis and therapy of Gram-positive bacteria keratitis.
基金the National Natural Science Foundation of China(Nos.21825402,31400860,22393932,T2321005,and 22204117)the Science and Technology Development Fund,Macao SAR(Nos.0002/2022/AKP and 0115/2023/RIA2)+3 种基金the National Key R&D Program of China(No.2023YFB3208200)the Natural Science Foundation of Jiangsu Province of China(Nos.BK20191417 and BK20170061)the Program for Jiangsu Specially Appointed Professors to Y.H.,a project funded by the Priority Academic Program Development of Jiangsu Higher Education Institutions(PAPD)111 Project and Collaborative Innovation Center of Suzhou Nano Science and Technology(NANO-CIC).
文摘Despite sufficient studies performed in non-primate animal models,there exists scanty information obtained from pilot trials in non-human primate animal models,severely hindering nanomaterials moving from basic research into clinical practice.We herein present a pioneering demonstration of nanomaterials based optical imaging-guided surgical operation by using macaques as a typical kind of non-human primate-animal models.Typically,taking advantages of strong and stable fluorescence of the small-sized(diameter:~5 nm)silicon-based nanoparticles(SiNPs),lymphatic drainage patterns can be vividly visualized in a real-time manner,and lymph nodes(LN)are able to be sensitively detected and precisely excised from small animal models(e.g.,rats and rabbits)to non-human primate animal models(e.g.,cynomolgus macaque(Macaca fascicularis)and rhesus macaque(Macaca mulatta)).Compared to clinically used invisible near-infrared(NIR)lymphatic tracers(i.e.,indocyanine green(ICG);etc.),we fully indicate that the SiNPs feature unique advantages for naked-eye visible fluorescence-guided surgical operation in long-term manners.Thorough toxicological analysis in macaque models further provides confirming evidence of favorable biocompatibility of the SiNPs probes.We expect that our findings would facilitate the translation of nanomaterials from the laboratory to the clinic,especially in the field of cancer treatment.
