Dear Editor,We are writing to present a case of bilateral sequential cytomegalovirus retinitis(CMVR)in a patient with dyskeratosis congenita(DC).DC is an inherited bone marrow failure syndrome characterized by a typic...Dear Editor,We are writing to present a case of bilateral sequential cytomegalovirus retinitis(CMVR)in a patient with dyskeratosis congenita(DC).DC is an inherited bone marrow failure syndrome characterized by a typical diagnostic triad of oral leukoplakia,nail dystrophy,and reticular hyperpigmentation[1].DC can be inherited in one of the three forms,X-linked,autosomal dominant,and autosomal recessive.展开更多
Blueberries are a rich source of anthocyanins, which are associated with health benefits contributing to a reduced risk for many diseases. The present study identified the functional Gardenblue blueberry （Vaccinium a...Blueberries are a rich source of anthocyanins, which are associated with health benefits contributing to a reduced risk for many diseases. The present study identified the functional Gardenblue blueberry （Vaccinium ashei Reade） anthocyanin extracts （GBBAEs） and evaluated their capacity and underlying mechanisms in protecting murine RAW 264.7 cells from lipopolysaccharide （LPS）-stimulated inflammation in vitro. Enzyme-linked immunosorbent assay （ELISA） kit results showed that GBBAEs significantly inhibited the production of nitric oxide （NO）, prostaglandin E2 （PGE2）, interleukin-6 （IL-6）, IL-113, and interferon-y （INF-y）. Real-time polymerase chain reaction （PCR） analysis in- dicated that the mRNA expression levels of IL-6, IL-113, tumor necrosis factor-α （TNF-α）, monocyte chemoattractant protein-1 （MCP-1）, and cyclooxygenase 2 （COX-2） were suppressed in LPS-stimulated RAW 264.7 cells. Additionally, Western blot analysis was used to evaluate the relative protein expression levels of COX-2 and nuclear factor-κB p65 （NF-κBp65）. All these results suggested the potential use of GBBAEs as a functional food for the treatment of in- flammatory diseases.展开更多
Soxhlet extraction is a common method of sample preparation. However, there has been no discussion about the efficiency of Soxhlet extraction from different batches and the factors that cause content fluctuation. In t...Soxhlet extraction is a common method of sample preparation. However, there has been no discussion about the efficiency of Soxhlet extraction from different batches and the factors that cause content fluctuation. In this study, Panax ginseng was selected as a model sample. Soxhlet extraction by means of a water bath, which has always been neglected, was identified as a novel key factor in the poor repeat- ability in different batches of Soxhlet extraction, as it can affect the siphon times and reflux time, which have been positively correlated with the ginsenoside contents. By substituting round bottom flasks in the same column, the relative standard deviation of the most fluctuated compound, ginsenoside Rbl, was decreased from 24.6% to 5.02%. Scanning electron microscopy analysis confirmed that the breakdown of the surface of the ginseng powder in the Soxhlet extraction led to a better dissolution of ginsenosides, indicating that chloroform may promote the extraction of ginsenosides by disrupting the cell structure. Moreover, 70% methanol was regarded as the better solvent for extracting the ginsenosides. Overall, this work offers a practical and effective protocol for improving the accuracy and repeatability of Soxhlet extraction methodology for ginsenosides and other analytes.展开更多
The method of variable separation has always been regarded as a crucial method for solving nonlinear evolution equations.In this paper,we use a new form of variable separation to study novel soliton molecules and thei...The method of variable separation has always been regarded as a crucial method for solving nonlinear evolution equations.In this paper,we use a new form of variable separation to study novel soliton molecules and their interactions in(2+1)-dimensional potential Boiti–Leon-Manna–Pempinelli equation.Dromion molecules,ring molecules,lump molecules,multi-instantaneous molecules,and their interactions are obtained.Then we draw corresponding images with maple software to study their dynamic behavior.展开更多
BACKGROUND Familial hypercholesterolemia(FH)is a common autosomal dominant hereditary disease.Its early diagnosis and intervention significantly improve the patient’s quality of life.However,there are few types of re...BACKGROUND Familial hypercholesterolemia(FH)is a common autosomal dominant hereditary disease.Its early diagnosis and intervention significantly improve the patient’s quality of life.However,there are few types of research on the FH pathogenic genes in China.METHODS In this study,we recruited a family diagnosed with FH and used whole exome sequencing(WES)to analyze the proband variants.Intracellular cholesterol level,reactive oxygen species(ROS)level,and the expression of pyroptosis-related genes were detected after overexpression of wild-type or variant LDLR in L02 cells.RESULTS A heterozygous missense variant predicted to be deleterious to LDLR(c.1879G>A,p.Ala627Thr)was identified in the proband.Mechanistically,intracellular cholesterol level,ROS level,and the expression of pyroptosis-related genes,nucleotidebinding oligomerization domain-like receptor family protein 3(NLRP3)inflammasome and components(caspase 1,apoptosis-associated speck-like protein containing a caspase recruitment domain(ASC)and NLRP3),gasdermin D(GSDMD),interleukin(IL)-18,IL-1βwas elevated in the variant LDLR group,which was attenuated by inhibition of ROS.CONCLUSIONS FH is associated with a variant(c.1879G>A,p.Ala627Thr)in the LDLR gene.Regarding the mechanism,the ROS/NLRP3-mediated pyroptosis in hepatic cells may contribute to the pathogenesis of the LDLR variant.展开更多
Background:Dengue cases have been reported each year for the past 25 years in Guangdong Province,China with a recorded historical peak in 2014.This study aims to describe the epidemiological characteristics of this la...Background:Dengue cases have been reported each year for the past 25 years in Guangdong Province,China with a recorded historical peak in 2014.This study aims to describe the epidemiological characteristics of this large outbreak in order to better understand its epidemic factors and to inform control strategies.Methods:Data for clinically diagnosed and laboratory-confirmed dengue fever cases in 2014 were extracted from the China Notifiable Infectious Disease Reporting System.We analyzed the incidence and characteristics of imported and indigenous cases in terms of population,temporal and spatial distributions.Results:A total of 45224 dengue fever cases and 6 deaths were notified in Guangdong Province in 2014,with an incidence of 47.3 per 100000 people.The elderly(65+years)represented 11.7%of total indigenous cases with the highest incidence(72.3 per 100000).Household workers and the unemployed accounted for 23.1%of indigenous cases.The majority of indigenous cases occurred in the 37th to 44th week of 2014(September and October)and almost all(20 of 21)prefecture-level cities in Guangdong were affected.Compared to the non-Pearl River Delta Region,the Pearl River Delta Region accounted for the majority of dengue cases and reported cases earlier in 2014.Dengue virus serotypes 1(DENV-1),2(DENV-2)and 3(DENV-3)were detected and DENV-1 was predominant(88.4%).Conclusions:Dengue fever is a serious public health problem and is emerging as a continuous threat in Guangdong Province.There is an urgent need to enhance dengue surveillance and control,especially for the high-risk populations in high-risk areas.展开更多
Background:Dengue fever(DF)is a mosquito-borne infectious disease that has threatened tropical and subtropical regions in recent decades.An early and targeted warning of a dengue epidemic is important for vector contr...Background:Dengue fever(DF)is a mosquito-borne infectious disease that has threatened tropical and subtropical regions in recent decades.An early and targeted warning of a dengue epidemic is important for vector control.Current studies have primarily determined weather conditions to be the main factor for dengue forecasting,thereby neglecting that environmental suitability for mosquito breeding is also an important factor,especially in fine-grained intra-urban settings.Considering that street-view images are promising for depicting physical environments,this study proposes a framework for facilitating fine-grained intra-urban dengue forecasting by integrating the urban environments measured from street-view images.展开更多
Ischemic stroke represents a significant danger to human beings,especially the elderly.Interventions are only available to remove the clot,and the mechanism of neuronal death during ischemic stroke is still in debate....Ischemic stroke represents a significant danger to human beings,especially the elderly.Interventions are only available to remove the clot,and the mechanism of neuronal death during ischemic stroke is still in debate.Ferroptosis is increasingly appreciated as a mechanism of cell death after ischemia in various organs.展开更多
Based on the homogeneous balance method, the Jacobi elliptic expansion method and the auxiliary equation method, the first elliptic function equation is used to get a new kind of solutions of nonlinear evolution equat...Based on the homogeneous balance method, the Jacobi elliptic expansion method and the auxiliary equation method, the first elliptic function equation is used to get a new kind of solutions of nonlinear evolution equations. New exact solutions to the Jacobi elliptic function of MKdV equations and Benjamin-Bona-Mahoney （BBM） equations are obtained with the aid of computer algebraic system Maple. The method is also valid for other （l＋l）-dimensional and higher dimensional systems.展开更多
基金Supported by grants from the Basic Research Project of Yunnan Province(No.2018NS0273)the Medical Reserve Talents Training Program of Yunnan Provincial Health Commission(No.H-2018021)the Yunnan Talent Support Plan-Young Talent Program(No.XDYCQNRC-2022-0702).
文摘Dear Editor,We are writing to present a case of bilateral sequential cytomegalovirus retinitis(CMVR)in a patient with dyskeratosis congenita(DC).DC is an inherited bone marrow failure syndrome characterized by a typical diagnostic triad of oral leukoplakia,nail dystrophy,and reticular hyperpigmentation[1].DC can be inherited in one of the three forms,X-linked,autosomal dominant,and autosomal recessive.
基金Project supported by the Fundamental Research Funds for the Central Universities of China(No.2013PY095)the Clinical Research Project of Health and Family Planning Commission of Wuhan Municipality(No.WX13A05)+1 种基金the Research Project of Wuhan City Central Hospital(No.YQ15A04)the Grant from Key Laboratory of Biological Targeted Therapy of Hubei Province of China(No.02.03.2014-10)
文摘Blueberries are a rich source of anthocyanins, which are associated with health benefits contributing to a reduced risk for many diseases. The present study identified the functional Gardenblue blueberry （Vaccinium ashei Reade） anthocyanin extracts （GBBAEs） and evaluated their capacity and underlying mechanisms in protecting murine RAW 264.7 cells from lipopolysaccharide （LPS）-stimulated inflammation in vitro. Enzyme-linked immunosorbent assay （ELISA） kit results showed that GBBAEs significantly inhibited the production of nitric oxide （NO）, prostaglandin E2 （PGE2）, interleukin-6 （IL-6）, IL-113, and interferon-y （INF-y）. Real-time polymerase chain reaction （PCR） analysis in- dicated that the mRNA expression levels of IL-6, IL-113, tumor necrosis factor-α （TNF-α）, monocyte chemoattractant protein-1 （MCP-1）, and cyclooxygenase 2 （COX-2） were suppressed in LPS-stimulated RAW 264.7 cells. Additionally, Western blot analysis was used to evaluate the relative protein expression levels of COX-2 and nuclear factor-κB p65 （NF-κBp65）. All these results suggested the potential use of GBBAEs as a functional food for the treatment of in- flammatory diseases.
基金financial support of the key project at the central government level:The Ability Establishment of Sustainable Use for Valuable Chinese Medicine Resources(No.2060302)the Independent Research Grant of National Resource Center for Chinese Materia Medica,China Academy of Chinese Medical Sciences(No.ZZXT201608)the National Natural Science Foundation of China(No.81603293)
文摘Soxhlet extraction is a common method of sample preparation. However, there has been no discussion about the efficiency of Soxhlet extraction from different batches and the factors that cause content fluctuation. In this study, Panax ginseng was selected as a model sample. Soxhlet extraction by means of a water bath, which has always been neglected, was identified as a novel key factor in the poor repeat- ability in different batches of Soxhlet extraction, as it can affect the siphon times and reflux time, which have been positively correlated with the ginsenoside contents. By substituting round bottom flasks in the same column, the relative standard deviation of the most fluctuated compound, ginsenoside Rbl, was decreased from 24.6% to 5.02%. Scanning electron microscopy analysis confirmed that the breakdown of the surface of the ginseng powder in the Soxhlet extraction led to a better dissolution of ginsenosides, indicating that chloroform may promote the extraction of ginsenosides by disrupting the cell structure. Moreover, 70% methanol was regarded as the better solvent for extracting the ginsenosides. Overall, this work offers a practical and effective protocol for improving the accuracy and repeatability of Soxhlet extraction methodology for ginsenosides and other analytes.
基金the National Natural Science Foundation of China(Grant Nos.11371086,11671258,and 11975145)the Fund of Science and Technology Commission of Shanghai Municipality(Grant No.13ZR1400100)。
文摘The method of variable separation has always been regarded as a crucial method for solving nonlinear evolution equations.In this paper,we use a new form of variable separation to study novel soliton molecules and their interactions in(2+1)-dimensional potential Boiti–Leon-Manna–Pempinelli equation.Dromion molecules,ring molecules,lump molecules,multi-instantaneous molecules,and their interactions are obtained.Then we draw corresponding images with maple software to study their dynamic behavior.
基金supported by the Health and Family Planning Commission of Wuhan City(Grant Number WX18M02)Central Guiding Local Science and Technology Development Special Project(No.2022BGE272).
文摘BACKGROUND Familial hypercholesterolemia(FH)is a common autosomal dominant hereditary disease.Its early diagnosis and intervention significantly improve the patient’s quality of life.However,there are few types of research on the FH pathogenic genes in China.METHODS In this study,we recruited a family diagnosed with FH and used whole exome sequencing(WES)to analyze the proband variants.Intracellular cholesterol level,reactive oxygen species(ROS)level,and the expression of pyroptosis-related genes were detected after overexpression of wild-type or variant LDLR in L02 cells.RESULTS A heterozygous missense variant predicted to be deleterious to LDLR(c.1879G>A,p.Ala627Thr)was identified in the proband.Mechanistically,intracellular cholesterol level,ROS level,and the expression of pyroptosis-related genes,nucleotidebinding oligomerization domain-like receptor family protein 3(NLRP3)inflammasome and components(caspase 1,apoptosis-associated speck-like protein containing a caspase recruitment domain(ASC)and NLRP3),gasdermin D(GSDMD),interleukin(IL)-18,IL-1βwas elevated in the variant LDLR group,which was attenuated by inhibition of ROS.CONCLUSIONS FH is associated with a variant(c.1879G>A,p.Ala627Thr)in the LDLR gene.Regarding the mechanism,the ROS/NLRP3-mediated pyroptosis in hepatic cells may contribute to the pathogenesis of the LDLR variant.
基金supported by Guangdong Provincial Science and Technology Project Fundings(NO.2013A020229005,NO.2014A040401041,NO.2015A020213004).
文摘Background:Dengue cases have been reported each year for the past 25 years in Guangdong Province,China with a recorded historical peak in 2014.This study aims to describe the epidemiological characteristics of this large outbreak in order to better understand its epidemic factors and to inform control strategies.Methods:Data for clinically diagnosed and laboratory-confirmed dengue fever cases in 2014 were extracted from the China Notifiable Infectious Disease Reporting System.We analyzed the incidence and characteristics of imported and indigenous cases in terms of population,temporal and spatial distributions.Results:A total of 45224 dengue fever cases and 6 deaths were notified in Guangdong Province in 2014,with an incidence of 47.3 per 100000 people.The elderly(65+years)represented 11.7%of total indigenous cases with the highest incidence(72.3 per 100000).Household workers and the unemployed accounted for 23.1%of indigenous cases.The majority of indigenous cases occurred in the 37th to 44th week of 2014(September and October)and almost all(20 of 21)prefecture-level cities in Guangdong were affected.Compared to the non-Pearl River Delta Region,the Pearl River Delta Region accounted for the majority of dengue cases and reported cases earlier in 2014.Dengue virus serotypes 1(DENV-1),2(DENV-2)and 3(DENV-3)were detected and DENV-1 was predominant(88.4%).Conclusions:Dengue fever is a serious public health problem and is emerging as a continuous threat in Guangdong Province.There is an urgent need to enhance dengue surveillance and control,especially for the high-risk populations in high-risk areas.
文摘Background:Dengue fever(DF)is a mosquito-borne infectious disease that has threatened tropical and subtropical regions in recent decades.An early and targeted warning of a dengue epidemic is important for vector control.Current studies have primarily determined weather conditions to be the main factor for dengue forecasting,thereby neglecting that environmental suitability for mosquito breeding is also an important factor,especially in fine-grained intra-urban settings.Considering that street-view images are promising for depicting physical environments,this study proposes a framework for facilitating fine-grained intra-urban dengue forecasting by integrating the urban environments measured from street-view images.
基金This work was supported by the National Key Research and Development Project of China(2018YFC1312300)the National Natural Science Foundation of China(81722016,81801182)+2 种基金he program of National Clinical Research Center for Geriatrics of West China Hospital(Z2021LC001,Z20191001)West China Hospital 1.3.5 project for disciplines of excellence(ZYYC20007,ZYYC20009)Sichuan University postdoctoral interdisciplinary Innovation Fund.
文摘Ischemic stroke represents a significant danger to human beings,especially the elderly.Interventions are only available to remove the clot,and the mechanism of neuronal death during ischemic stroke is still in debate.Ferroptosis is increasingly appreciated as a mechanism of cell death after ischemia in various organs.
基金Supported by the National Natural Science Foundation of China (No. 10647112)the Foundation of Donghua University
文摘Based on the homogeneous balance method, the Jacobi elliptic expansion method and the auxiliary equation method, the first elliptic function equation is used to get a new kind of solutions of nonlinear evolution equations. New exact solutions to the Jacobi elliptic function of MKdV equations and Benjamin-Bona-Mahoney （BBM） equations are obtained with the aid of computer algebraic system Maple. The method is also valid for other （l＋l）-dimensional and higher dimensional systems.
