Oleaginous fungi can convert a variety of substrates into lipids,frequently containing(20-70%,w/w)of their cell dry weight(CDW),which positions them as promising candidates for sustainable lipid production.However,the...Oleaginous fungi can convert a variety of substrates into lipids,frequently containing(20-70%,w/w)of their cell dry weight(CDW),which positions them as promising candidates for sustainable lipid production.However,the interplay of calcium(Ca^(2+))in regulating lipid accumulation remains unclear.Therefore,the effect of different Ca^(2+)concentrations on the oleaginous capacity of Mucor circinelloides strain WJ11 was evaluated.Hence,WJ11 was fermented for 96 h using different Ca^(2+)levels.Maximum CDW(17.2 g/L)and lipid yield(42%,w/w)were achieved at 2.5 and 0.3 g/L Ca^(2+),respectively,compared to 15.8 g/L CDW and(34%,w/w)lipid content in the control(0.1 g/L Ca^(2+)).High Ca^(2+)levels(12.5-20 g/L)improved the production of certain fatty acids(FAs),whereas optimal levels favoured the accumulation of stearic acid(C18:0)andγ-linolenic acid(C18:3)at designated time intervals.Furthermore,the calcineurin catalytic subunits(CnaA,CnaB,and CnaC)and regulatory subunit(CAR1)were significantly upregulated after 12 h of fermentation.Additionally,lipogenic enzyme activity analysis of key genes involved in lipid biosynthesis exhibited upregulation at different Ca^(2+)concentrations.These findings indicated that raised Ca^(2+)levels can significantly boost lipid production in industrial fermentations.This study is the first to investigate the role of Ca^(2+)in the oleaginous fungus M.circinelloides,providing new avenues into the integrated functions of Ca^(2+)and its signaling pathways in lipid metabolism,particularly in oleaginous fungi.展开更多
基金supported by the National Natural Science Foundation of China under Grant(No.31972851)the Shandong Province Small and Medium Enterprises Innovation Ability Enhancement Project(grant No.2021TSGC1281 and 2023TSGC0050)supporting this work through project number(TU-DSPP-2024-186).
文摘Oleaginous fungi can convert a variety of substrates into lipids,frequently containing(20-70%,w/w)of their cell dry weight(CDW),which positions them as promising candidates for sustainable lipid production.However,the interplay of calcium(Ca^(2+))in regulating lipid accumulation remains unclear.Therefore,the effect of different Ca^(2+)concentrations on the oleaginous capacity of Mucor circinelloides strain WJ11 was evaluated.Hence,WJ11 was fermented for 96 h using different Ca^(2+)levels.Maximum CDW(17.2 g/L)and lipid yield(42%,w/w)were achieved at 2.5 and 0.3 g/L Ca^(2+),respectively,compared to 15.8 g/L CDW and(34%,w/w)lipid content in the control(0.1 g/L Ca^(2+)).High Ca^(2+)levels(12.5-20 g/L)improved the production of certain fatty acids(FAs),whereas optimal levels favoured the accumulation of stearic acid(C18:0)andγ-linolenic acid(C18:3)at designated time intervals.Furthermore,the calcineurin catalytic subunits(CnaA,CnaB,and CnaC)and regulatory subunit(CAR1)were significantly upregulated after 12 h of fermentation.Additionally,lipogenic enzyme activity analysis of key genes involved in lipid biosynthesis exhibited upregulation at different Ca^(2+)concentrations.These findings indicated that raised Ca^(2+)levels can significantly boost lipid production in industrial fermentations.This study is the first to investigate the role of Ca^(2+)in the oleaginous fungus M.circinelloides,providing new avenues into the integrated functions of Ca^(2+)and its signaling pathways in lipid metabolism,particularly in oleaginous fungi.
