Optical imaging techniques provide low-cost,non-radiative images with high spatiotemporal resolution,making them advantageous for long-term dynamic observation of blood perfusion in stroke research and other brain stu...Optical imaging techniques provide low-cost,non-radiative images with high spatiotemporal resolution,making them advantageous for long-term dynamic observation of blood perfusion in stroke research and other brain studies compared to non-optical methods.However,high-resolution imaging in optical microscopy fundamentally requires a tight optical focus,and thus a limited depth of field(DOF).Consequently,large-scale,non-stitched,high-resolution images of curved surfaces,like brains,are difficult to acquire without z-axis scanning.To overcome this limitation,we developed a needle-shaped beam optical coherence tomography angiography(NB-OCTA)system,and for the first time,achieved a volumetric resolution of less than 8μm in a non-stitched volume space of 6.4 mm×4 mm×620μm in vivo.This system captures the distribution of blood vessels at 3.4-times larger depths than normal OCTA equipped with a Gaussian beam(GB-OCTA).We then employed NB-OCTA to perform long-term observation of cortical blood perfusion after stroke in vivo,and quantitatively analyzed the vessel area density(VAD)and the diameters of representative vessels in different regions over 10 days,revealing different spatiotemporal dynamics in the acute,sub-acute and chronic phase of post-ischemic revascularization.Benefiting from our NB-OCTA,we revealed that the recovery process is not only the result of spontaneous reperfusion,but also the formation of new vessels.This study provides visual and mechanistic insights into strokes and helps to deepen our understanding of the spontaneous response of brain after stroke.展开更多
基金supported by the National Key R&D Program of China(No.2022YFB4702902)National Natural Science Foundation of China(Nos.61831014,62275023,and 32021002)+2 种基金Beijing Municipal Natural Science Foundation(No.4232077)Overseas Expertise Introduction Project for Discipline Innovation(No.B18005)STI2030-Major Projects(No.2022ZD0212000).
文摘Optical imaging techniques provide low-cost,non-radiative images with high spatiotemporal resolution,making them advantageous for long-term dynamic observation of blood perfusion in stroke research and other brain studies compared to non-optical methods.However,high-resolution imaging in optical microscopy fundamentally requires a tight optical focus,and thus a limited depth of field(DOF).Consequently,large-scale,non-stitched,high-resolution images of curved surfaces,like brains,are difficult to acquire without z-axis scanning.To overcome this limitation,we developed a needle-shaped beam optical coherence tomography angiography(NB-OCTA)system,and for the first time,achieved a volumetric resolution of less than 8μm in a non-stitched volume space of 6.4 mm×4 mm×620μm in vivo.This system captures the distribution of blood vessels at 3.4-times larger depths than normal OCTA equipped with a Gaussian beam(GB-OCTA).We then employed NB-OCTA to perform long-term observation of cortical blood perfusion after stroke in vivo,and quantitatively analyzed the vessel area density(VAD)and the diameters of representative vessels in different regions over 10 days,revealing different spatiotemporal dynamics in the acute,sub-acute and chronic phase of post-ischemic revascularization.Benefiting from our NB-OCTA,we revealed that the recovery process is not only the result of spontaneous reperfusion,but also the formation of new vessels.This study provides visual and mechanistic insights into strokes and helps to deepen our understanding of the spontaneous response of brain after stroke.
