Defective CD39 levels contribute to an imbalance between Tregs and Th17 effectors in inflammatory bowel disease(IBD).CD39 initiates an ATP hydrolysis cascade that culminates with the generation of adenosine,an immune ...Defective CD39 levels contribute to an imbalance between Tregs and Th17 effectors in inflammatory bowel disease(IBD).CD39 initiates an ATP hydrolysis cascade that culminates with the generation of adenosine,an immune metabolite that is key to tissue homeostasis.Human CD39 is regulated by an endogenous antisense RNA(CD39-AS)that is markedly elevated in IBD Tregs and Th17 cells.In this study,we investigated how CD39-AS affects the function of Tregs and Th17 cells in healthy subjects and IBD patients.We report that CD39-AS RNA is present in two main splice variants that are specifically expressed by Tregs or Th17 cells.Blockade of CD39-AS via self-delivering oligonucleotides targeting the splice variant expressed in Tregs results in a decrease of glucose transport and glycolysis and in enhanced Treg function and stability in IBD.In Th17 cells,silencing of CD39-AS limits oxidative responses and ameliorates mitochondrial health.These metabolic effects are also noted in a model of experimental colitis in humanized mice,along with reduced disease activity.Thus,in vivo administration of oligonucleotides targeting the Treg or Th17 cell CD39-AS variant limits disease activity,decreases the expression of GLUT1 and improves mitochondrial health in gut-derived CD4 lymphocytes.Mechanistically,activation of HIF-1αand STAT3 results in the upregulation of CD39-AS in IBD cells.In conclusion,CD39-AS is an important modulator of Treg and Th17 cell metabolism.Interference with this antisense RNA,or the factors favoring its upregulation,might contain inflammation and halt disease progression in IBD by restoring immune metabolism and Treg functional stability.展开更多
基金supported by the National Institutes of Health(R01DK124408 to MSL,R01GM135377 to SKK)the Crohn’s and Colitis Foundation(Litwin IBD Pioneers Award to MSL)+1 种基金Boehringer Ingelheim Fonds MD fellowship(to DN)grant APVV-21-0370(to BG).
文摘Defective CD39 levels contribute to an imbalance between Tregs and Th17 effectors in inflammatory bowel disease(IBD).CD39 initiates an ATP hydrolysis cascade that culminates with the generation of adenosine,an immune metabolite that is key to tissue homeostasis.Human CD39 is regulated by an endogenous antisense RNA(CD39-AS)that is markedly elevated in IBD Tregs and Th17 cells.In this study,we investigated how CD39-AS affects the function of Tregs and Th17 cells in healthy subjects and IBD patients.We report that CD39-AS RNA is present in two main splice variants that are specifically expressed by Tregs or Th17 cells.Blockade of CD39-AS via self-delivering oligonucleotides targeting the splice variant expressed in Tregs results in a decrease of glucose transport and glycolysis and in enhanced Treg function and stability in IBD.In Th17 cells,silencing of CD39-AS limits oxidative responses and ameliorates mitochondrial health.These metabolic effects are also noted in a model of experimental colitis in humanized mice,along with reduced disease activity.Thus,in vivo administration of oligonucleotides targeting the Treg or Th17 cell CD39-AS variant limits disease activity,decreases the expression of GLUT1 and improves mitochondrial health in gut-derived CD4 lymphocytes.Mechanistically,activation of HIF-1αand STAT3 results in the upregulation of CD39-AS in IBD cells.In conclusion,CD39-AS is an important modulator of Treg and Th17 cell metabolism.Interference with this antisense RNA,or the factors favoring its upregulation,might contain inflammation and halt disease progression in IBD by restoring immune metabolism and Treg functional stability.
