Fluorescent probes have emerged as indispensable chemical tools to the field of chemical biology and medicine.The ability to detect intracellular species and monitor physiological processes has not only advanced our k...Fluorescent probes have emerged as indispensable chemical tools to the field of chemical biology and medicine.The ability to detect intracellular species and monitor physiological processes has not only advanced our knowledge in biology but has provided new approaches towards disease diagnosis.In this review,we detail the design criteria and strategies for some recently reported fluorescent probes that can detect a wide range of biologically important species in cells and in vivo.In doing so,we highlight the importance of each biological species and their role in biological systems and for disease progression.We then discuss the current problems and challenges of existing technologies and provide our perspective on the future directions of the research area.Overall,we hope this review will provide inspiration for researchers and prove as useful guide for the development of the next generation of fluorescent probes.展开更多
An increased demand for iron is a hallmark of cancer cells and is thought necessary to promote high cell proliferation,tumor progression and metastasis.This makes iron metabolism an attractive therapeutic target.Unfor...An increased demand for iron is a hallmark of cancer cells and is thought necessary to promote high cell proliferation,tumor progression and metastasis.This makes iron metabolism an attractive therapeutic target.Unfortunately,current iron-based therapeutic strategies often lack effectiveness and can elicit off-target toxicities.We report here a dual-therapeutic prodrug,DOXjade,that allows for iron chelation chemo-photothermal cancer therapy.This prodrug takes advantage of the clinically approved iron chelator deferasirox(ExJade®)and the topoisomerase 2 inhibitor,doxorubicin(DOX).Loading DOXjade onto ultrathin 2D Ti_(3)C_(2) MXene nanosheets produces a construct,Ti_(3)C_(2)-PVP@DOXjade,that allows the iron chelation and chemotherapeutic functions of DOXjade to be photo-activated at the tumor sites,while potentiating a robust photothermal effect with photothermal conversion efficiencies of up to 40%.Antitumor mechanistic investigations reveal that upon activation,Ti_(3)C_(2)-PVP@DOXjade serves to promote apoptotic cell death and downregulate the iron depletion-induced iron transferrin receptor(TfR).A tumor pH-responsive iron chelation/photothermal/chemotherapy antitumor effect was achieved both in vitro and in vivo.The results of this study highlight what may constitute a promising iron chelation-based phototherapeutic approach to cancer therapy.展开更多
A simple dual analyte fluorescein-based probe(PF3-Glc)was synthesised containingβ-glucosidase(β-glc)and hydrogen peroxide(H2O2)trigger units.The presence ofβ-glc,resulted in fragmentation of the parent molecule rel...A simple dual analyte fluorescein-based probe(PF3-Glc)was synthesised containingβ-glucosidase(β-glc)and hydrogen peroxide(H2O2)trigger units.The presence ofβ-glc,resulted in fragmentation of the parent molecule releasing glucose and the slightly fluorescent mono-boronate fluorescein(PF3).Subsequently,in the presence of glucose oxidase(GOx),the released glucose was catalytically converted to D-glucono-δ-lactone,which produced H2O2 as a by-product.The GOx-produced H2O2,resulted in classic H2O2-mediated boronate oxidation and the release of the highly emissive fluorophore,fluorescein.This unique cascade reaction lead to an 80-fold increase in fluorescence intensity.展开更多
A boronic acid-based anthracene fluorescent probe was functionalised with an acrylamide unit to incorporate into a hydrogel system for monosaccharide detection.In solution,the fluorescent probe displayed a strong fluo...A boronic acid-based anthracene fluorescent probe was functionalised with an acrylamide unit to incorporate into a hydrogel system for monosaccharide detection.In solution,the fluorescent probe displayed a strong fluorescence turn-on response upon exposure to fructose,and an expected trend in apparent binding constants,as judged by a fluorescence response where D-fructose>D-galactose>D-mannose>D-glucose.The hydrogel incorporating the boronic acid monomer demonstrated the ability to detect monosaccharides by fluorescence with the same overall trend as the monomer in solution with the addition of D-fructose resulting in a 10-fold enhancement(≤0.25 mol/L).展开更多
We describe the synthesis and evaluation of an azulene-based chemodosimeter for nitrite.The probe was found to undergo two distinct color changes upon introduction of aqueous nitrite ion.A near-instant formation of a ...We describe the synthesis and evaluation of an azulene-based chemodosimeter for nitrite.The probe was found to undergo two distinct color changes upon introduction of aqueous nitrite ion.A near-instant formation of a grey color provides a qualitative indication of the presence of nitrite,followed by the formation of a deep-yellow/orange color,the endpoint from which quantitative data can be derived.The azulene probe exhibits 1:1 stoichiometry of reaction with nitrite in water,and is selective for nitrite over other anions.The azulene probe was applied to determine nitrite content in cured meat,and compared with the British Standard testing procedure(Griess test).The value obtained from the azulene-based probe agreed closely with the standard test.Our procedure only requires the preparation of one standard solution,instead of the three required for the standard Griess test.展开更多
Fluorescent probes are intelligently designed molecules that transform the act of binding/reacting with a target analyte into an optical signal[1,2].Their high sensitivity and specificity coupled with high spatial and...Fluorescent probes are intelligently designed molecules that transform the act of binding/reacting with a target analyte into an optical signal[1,2].Their high sensitivity and specificity coupled with high spatial and temporal resolution offers a non-invasive approach to observe a specific analyte within complex biological environments in real time with high precision[3].These"molecular tools"have the capability to interrogate the role of specific biomarkers that are associated with a particular disease state or can be used to improve our understanding of the role or certain species in a biological pathway[4,5].展开更多
基金the National Natural Science Foundation of China(21788102,91853201)the Shanghai Municipal Science and Technology Major Project(2018SHZDZX03)+9 种基金the International Cooperation Program of Shanghai Science and Technology Committee(17520750100)the Projects from the Shanghai Science and Techonology Commission(19441905000)the Fundamental Research Funds for the Central Universities(222201717003)the Programme of Introducing Talents of Discipline to Universities(B16017)for financial supportthe National Natural Science Foundation of China(22107028)National Postdoctoral Program for Innovative Talents(BX20190115)Shanghai Post-doctoral Excellence Program(2019044)China Postdoctoral Science Foundation(2020M681206)for financial supportthe Project funded by China Postdoctoral Science Foundation(2020M681196)the Royal Society for a Wolfson Research Merit Award and the Open Research Fund of the School of Chemistry and Chemical Engineering,Henan Normal University for support(2020ZD01)。
文摘Fluorescent probes have emerged as indispensable chemical tools to the field of chemical biology and medicine.The ability to detect intracellular species and monitor physiological processes has not only advanced our knowledge in biology but has provided new approaches towards disease diagnosis.In this review,we detail the design criteria and strategies for some recently reported fluorescent probes that can detect a wide range of biologically important species in cells and in vivo.In doing so,we highlight the importance of each biological species and their role in biological systems and for disease progression.We then discuss the current problems and challenges of existing technologies and provide our perspective on the future directions of the research area.Overall,we hope this review will provide inspiration for researchers and prove as useful guide for the development of the next generation of fluorescent probes.
基金supported by the National Natural Science Foundation of China(Grant No.11904239,Y.W.W)the Creative Research Initiative of National Research Foundation of Korea(NRF)(CRI project No.2018R1A3B1052702,J.S.K.)+1 种基金Initial support for the work in Austin came from the National Institutes of Health(CA 68682 to J.L.S.)with subsequent funding from the Robert A.Welch Foundation(F-0018 to J.L.S.)supported by Brain Pool Program through the funded by the Ministry of Science and ICT(Grant No.2020H1D3A1A02080172,M.L.).
文摘An increased demand for iron is a hallmark of cancer cells and is thought necessary to promote high cell proliferation,tumor progression and metastasis.This makes iron metabolism an attractive therapeutic target.Unfortunately,current iron-based therapeutic strategies often lack effectiveness and can elicit off-target toxicities.We report here a dual-therapeutic prodrug,DOXjade,that allows for iron chelation chemo-photothermal cancer therapy.This prodrug takes advantage of the clinically approved iron chelator deferasirox(ExJade®)and the topoisomerase 2 inhibitor,doxorubicin(DOX).Loading DOXjade onto ultrathin 2D Ti_(3)C_(2) MXene nanosheets produces a construct,Ti_(3)C_(2)-PVP@DOXjade,that allows the iron chelation and chemotherapeutic functions of DOXjade to be photo-activated at the tumor sites,while potentiating a robust photothermal effect with photothermal conversion efficiencies of up to 40%.Antitumor mechanistic investigations reveal that upon activation,Ti_(3)C_(2)-PVP@DOXjade serves to promote apoptotic cell death and downregulate the iron depletion-induced iron transferrin receptor(TfR).A tumor pH-responsive iron chelation/photothermal/chemotherapy antitumor effect was achieved both in vitro and in vivo.The results of this study highlight what may constitute a promising iron chelation-based phototherapeutic approach to cancer therapy.
基金the EPSRC and the University of Bath for funding.
文摘A simple dual analyte fluorescein-based probe(PF3-Glc)was synthesised containingβ-glucosidase(β-glc)and hydrogen peroxide(H2O2)trigger units.The presence ofβ-glc,resulted in fragmentation of the parent molecule releasing glucose and the slightly fluorescent mono-boronate fluorescein(PF3).Subsequently,in the presence of glucose oxidase(GOx),the released glucose was catalytically converted to D-glucono-δ-lactone,which produced H2O2 as a by-product.The GOx-produced H2O2,resulted in classic H2O2-mediated boronate oxidation and the release of the highly emissive fluorophore,fluorescein.This unique cascade reaction lead to an 80-fold increase in fluorescence intensity.
基金grateful for the support of the EPSRC and DTI(DT/F00267X/1)the Leverhulme Trust for support(F00094BC)the JDRF(2-SRA-2016-267-A-N)for support.
文摘A boronic acid-based anthracene fluorescent probe was functionalised with an acrylamide unit to incorporate into a hydrogel system for monosaccharide detection.In solution,the fluorescent probe displayed a strong fluorescence turn-on response upon exposure to fructose,and an expected trend in apparent binding constants,as judged by a fluorescence response where D-fructose>D-galactose>D-mannose>D-glucose.The hydrogel incorporating the boronic acid monomer demonstrated the ability to detect monosaccharides by fluorescence with the same overall trend as the monomer in solution with the addition of D-fructose resulting in a 10-fold enhancement(≤0.25 mol/L).
基金grateful Ph.D.funding to C.M.L.-A.from the EU Horizon 2020 research and innovation programme under grant agreement H2020-MSCA-CO-FUND,#665992The Centre for Sustainable Chemical Technologies is supported by EPSRC under grant EP/L016354/1EPSRC for DTP Ph.D. funding to L.C.M.
文摘We describe the synthesis and evaluation of an azulene-based chemodosimeter for nitrite.The probe was found to undergo two distinct color changes upon introduction of aqueous nitrite ion.A near-instant formation of a grey color provides a qualitative indication of the presence of nitrite,followed by the formation of a deep-yellow/orange color,the endpoint from which quantitative data can be derived.The azulene probe exhibits 1:1 stoichiometry of reaction with nitrite in water,and is selective for nitrite over other anions.The azulene probe was applied to determine nitrite content in cured meat,and compared with the British Standard testing procedure(Griess test).The value obtained from the azulene-based probe agreed closely with the standard test.Our procedure only requires the preparation of one standard solution,instead of the three required for the standard Griess test.
文摘Fluorescent probes are intelligently designed molecules that transform the act of binding/reacting with a target analyte into an optical signal[1,2].Their high sensitivity and specificity coupled with high spatial and temporal resolution offers a non-invasive approach to observe a specific analyte within complex biological environments in real time with high precision[3].These"molecular tools"have the capability to interrogate the role of specific biomarkers that are associated with a particular disease state or can be used to improve our understanding of the role or certain species in a biological pathway[4,5].
