Humanized mice are immunodeficient animals engrafted with human hematopoietic stem cells that give rise to various lineages of human blood cells throughout the life of the mouse. This article reviews recent advances i...Humanized mice are immunodeficient animals engrafted with human hematopoietic stem cells that give rise to various lineages of human blood cells throughout the life of the mouse. This article reviews recent advances in the generation of humanized mice, focusing on practical considerations. We discuss features of different immunodeficient recipient mouse strains, sources of human hematopoietic stem cells, advances in expansion and genetic modification of hematopoietic stem cells, and techniques to modulate the cytokine environment of recipient mice, in order to enhance reconstitution of specific human blood lineage cells. We highlight the opportunities created by new technologies and discuss practical considerations on how to make best use of the widening array of basic models for specific research applications.展开更多
Work recently published in PNAS by The Inflammation and Host Response to Injury, Large Scale Collaborative Research Program1 compared the transcriptional responses in peripheral blood to inflammatory injuries （burns,...Work recently published in PNAS by The Inflammation and Host Response to Injury, Large Scale Collaborative Research Program1 compared the transcriptional responses in peripheral blood to inflammatory injuries （burns, blunt force trauma） and to endotoxin in human patients and in corresponding mouse models.展开更多
文摘Humanized mice are immunodeficient animals engrafted with human hematopoietic stem cells that give rise to various lineages of human blood cells throughout the life of the mouse. This article reviews recent advances in the generation of humanized mice, focusing on practical considerations. We discuss features of different immunodeficient recipient mouse strains, sources of human hematopoietic stem cells, advances in expansion and genetic modification of hematopoietic stem cells, and techniques to modulate the cytokine environment of recipient mice, in order to enhance reconstitution of specific human blood lineage cells. We highlight the opportunities created by new technologies and discuss practical considerations on how to make best use of the widening array of basic models for specific research applications.
文摘Work recently published in PNAS by The Inflammation and Host Response to Injury, Large Scale Collaborative Research Program1 compared the transcriptional responses in peripheral blood to inflammatory injuries （burns, blunt force trauma） and to endotoxin in human patients and in corresponding mouse models.
