Objective: To evaluate in-vitro antioxidant, anti-inflammatory and antitumor abilities against human breast adenocarcinoma(MCF7) and human prostate cancer(PC3) as well as the suppressor effect of bacterial exopolysacc...Objective: To evaluate in-vitro antioxidant, anti-inflammatory and antitumor abilities against human breast adenocarcinoma(MCF7) and human prostate cancer(PC3) as well as the suppressor effect of bacterial exopolysaccharide(BAEPS) on Ehrlich ascites carcinoma(EAC). Methods: In-vitro antioxidants characters of BAEPS were determined using various methods, while anti-inflammatory activity was estimated against cyclooxygenase(COX-1 and COX-2). In-vitro study, anticancer against MCF7 and PC3 were assessed by the mitochondrial dependent reduction of yellow MTT. In in-vivo study against EAC progression, mice were inoculated with EAC cells and then were orally administered BAEPS at 200 mg/kg after 24 h(equals to 0.10 of determined LD50)/10 d. Results: BAEPS was acidic exopolysaccharide contained uronic acid(12.3%) and sulfate(22.8%) with constitution of glucose, galactose and glucuronic acid in a molar ratio1.6: 1.0: 0.9, respectively, with a molecular mass of 3.76伊104 g/mol. BAEPS appeared potent antioxidant characters as free radical scavenging, oxygen reactive species scavenging and metal chelation, while its reducing power was low. BAEPS showed selective anti-inflammatory activity against COX-2 than COX-1, COX-2 selective. BAEPS exhibited potent and selective effect to breast cell cancer MCF7, the death percentage was 65.20% with IC_(50)=70 μg/m L and IC_(90)=127.40 μg/m L. BAEPS decreased counted viable EAC cells and induced non-viable cells. BAEPS improved all assessed hematological parameters. These improvements were reflected in the increasing median survival time and significant increment(P<0.05) in life span. Conclusions: BAEPS has anti-tumor activity with a good margin of safety. The anti-tumor activity of BAEPS may be due to its content from sulfated groups and uronic acids and they have antioxidant and anti-inflammatory properties.展开更多
Objective:To study the antioxidant properties of crude extract of different Asteraceae plants.Methods:The antioxidant properties of six extracts were evaluated using different antioxidant tests,including free radical ...Objective:To study the antioxidant properties of crude extract of different Asteraceae plants.Methods:The antioxidant properties of six extracts were evaluated using different antioxidant tests,including free radical scavenging,reducing power,metal chelation,superoxide anion radical scavenging,total antioxidant capacity and inhibition of lipid peroxidation activities.Results:Picris cyanocarpa(P.cyanocarpa)and Anthemis deserti(A.deserti)had powerful antioxidant properties as radical scavenger,reducing agent and superoxide anion radical scavenger while Achillia fragrantissima(A.fragrantissima)and Artemissia monosperma(A.monosperma)were the most efficient as ion chelator(100%at 100,200 and 400μg/mL)A.fragrantissima and Rhanlarium appoposum(R.appoposum.)showed 100%inhibition on peroxidation of linoleic acid emulsion at 200 and 400μg/mL,while butylatedhydroxy toluene and ascorinc acid showed 100and 95%inhibition percentage at 400μg/mL,respectively.Those various antioxidant activities were compared to standard antioxidants such as butylated hydroxyl toluene and ascorbic acid.Conclusions:In most tests P.cyanocarpa and A.deserti had powerful antioxidant properties as radical scavenger,reducing agent and superoxide anion radical scavenger.展开更多
基金financially supported from National Research Centre,Egypt,through the Project No.10010103(2013-2016)
文摘Objective: To evaluate in-vitro antioxidant, anti-inflammatory and antitumor abilities against human breast adenocarcinoma(MCF7) and human prostate cancer(PC3) as well as the suppressor effect of bacterial exopolysaccharide(BAEPS) on Ehrlich ascites carcinoma(EAC). Methods: In-vitro antioxidants characters of BAEPS were determined using various methods, while anti-inflammatory activity was estimated against cyclooxygenase(COX-1 and COX-2). In-vitro study, anticancer against MCF7 and PC3 were assessed by the mitochondrial dependent reduction of yellow MTT. In in-vivo study against EAC progression, mice were inoculated with EAC cells and then were orally administered BAEPS at 200 mg/kg after 24 h(equals to 0.10 of determined LD50)/10 d. Results: BAEPS was acidic exopolysaccharide contained uronic acid(12.3%) and sulfate(22.8%) with constitution of glucose, galactose and glucuronic acid in a molar ratio1.6: 1.0: 0.9, respectively, with a molecular mass of 3.76伊104 g/mol. BAEPS appeared potent antioxidant characters as free radical scavenging, oxygen reactive species scavenging and metal chelation, while its reducing power was low. BAEPS showed selective anti-inflammatory activity against COX-2 than COX-1, COX-2 selective. BAEPS exhibited potent and selective effect to breast cell cancer MCF7, the death percentage was 65.20% with IC_(50)=70 μg/m L and IC_(90)=127.40 μg/m L. BAEPS decreased counted viable EAC cells and induced non-viable cells. BAEPS improved all assessed hematological parameters. These improvements were reflected in the increasing median survival time and significant increment(P<0.05) in life span. Conclusions: BAEPS has anti-tumor activity with a good margin of safety. The anti-tumor activity of BAEPS may be due to its content from sulfated groups and uronic acids and they have antioxidant and anti-inflammatory properties.
基金group no RGP-NPP-262 King Sand Universityprovided by the National Research Center,Cairo.Egypt
文摘Objective:To study the antioxidant properties of crude extract of different Asteraceae plants.Methods:The antioxidant properties of six extracts were evaluated using different antioxidant tests,including free radical scavenging,reducing power,metal chelation,superoxide anion radical scavenging,total antioxidant capacity and inhibition of lipid peroxidation activities.Results:Picris cyanocarpa(P.cyanocarpa)and Anthemis deserti(A.deserti)had powerful antioxidant properties as radical scavenger,reducing agent and superoxide anion radical scavenger while Achillia fragrantissima(A.fragrantissima)and Artemissia monosperma(A.monosperma)were the most efficient as ion chelator(100%at 100,200 and 400μg/mL)A.fragrantissima and Rhanlarium appoposum(R.appoposum.)showed 100%inhibition on peroxidation of linoleic acid emulsion at 200 and 400μg/mL,while butylatedhydroxy toluene and ascorinc acid showed 100and 95%inhibition percentage at 400μg/mL,respectively.Those various antioxidant activities were compared to standard antioxidants such as butylated hydroxyl toluene and ascorbic acid.Conclusions:In most tests P.cyanocarpa and A.deserti had powerful antioxidant properties as radical scavenger,reducing agent and superoxide anion radical scavenger.
