N6-methyladenosine methylation(m6A)is a common type of epigenetic alteration that prominently affects the prognosis of tumor patients.However,it is unknown how the m6A regulator affects the tumor microenvironment(TME)...N6-methyladenosine methylation(m6A)is a common type of epigenetic alteration that prominently affects the prognosis of tumor patients.However,it is unknown how the m6A regulator affects the tumor microenvironment(TME)cell infiltration in adrenocortical carcinoma(ACC)and how it affects the prognosis of ACC patients yet.The m6A alteration patterns of 112 ACC patients were evaluated,furthermore,the association with immune infiltration cell features was investigated.The unsupervised clustering method was applied to typify the m6A alteration patterns of ACC patients.The principal component analysis(PCA)technique was taken to create the m6A score to assess the alteration pattern in specific malignancies.We found two independent patterns of m6A alteration in ACC patients.The TME cell infiltration features were significantly in accordance with phenotypes of tumor immune-inflamed and immune desert in both patterns.The m6Ascore also served as an independent predictive factor in ACC patients.The somatic copy number variation(CNV)and patients prognosis can be predicted by m6A alteration patterns.Moreover,the ACC patients with high m6A scores had better overall survival(OS)and higher efficiency in immune checkpoint blockade therapy.Our work demonstrated the significance of m6A alteration to the ACC patients immunotherapy.The individual m6A alteration patterns analysis might contribute to ACC patients prognosis prediction and immunotherapy choice.展开更多
The effect of a machine feeding rate(FR;1,1.5 and 2 Mg/h)and/or three levels of selected fineness degree(FD;3,5 and 7 mm)on machine performance,pellet physical parameters,required energy and production cost of three m...The effect of a machine feeding rate(FR;1,1.5 and 2 Mg/h)and/or three levels of selected fineness degree(FD;3,5 and 7 mm)on machine performance,pellet physical parameters,required energy and production cost of three main types of broilers diets were examined in this experiment.The examined broiler diets were formulated to meet the Ross 308 strain requirements.A complete factorial design(3×3×3)was used to identify the effects of studied factors on the pellet mill machine and pellet production.The obtained results indicated that the pellet mill productivity significantly(p˂0.001)improved through increased pellet mill feeding rate level.In addition,the machine pelleting efficiency was found to be significantly affected by all studied variables and their interactions.While the total power consumption of the machine showed no variations under the impact of the tested factors or with any of their combinations.Regarding the pellet physical quality indices,all broiler diets with all selected FD and lower FR had the maximum durability and bulk density levels.Furthermore,lower feeding rates were associated with higher hardness degrees.The lowest production costs were substantially correlated with high FR and intermediate FD(5 mm).Furthermore,production costs were determined to be reduced in finisher broiler diets under different feeding rates.Moreover,manufacturing costs of finisher broiler meals were observed to decrease in several feeding rates.Overall,these findings indicate the capabilities of producing high-quality pellets and reducing the needed production costs by optimizing feeding rates to 2 Mg/h and 2 mm fineness in broiler diets.展开更多
基金Supporting Project Number(RSPD2023R725)King Saud University,Riyadh,Saud Arabia。
文摘N6-methyladenosine methylation(m6A)is a common type of epigenetic alteration that prominently affects the prognosis of tumor patients.However,it is unknown how the m6A regulator affects the tumor microenvironment(TME)cell infiltration in adrenocortical carcinoma(ACC)and how it affects the prognosis of ACC patients yet.The m6A alteration patterns of 112 ACC patients were evaluated,furthermore,the association with immune infiltration cell features was investigated.The unsupervised clustering method was applied to typify the m6A alteration patterns of ACC patients.The principal component analysis(PCA)technique was taken to create the m6A score to assess the alteration pattern in specific malignancies.We found two independent patterns of m6A alteration in ACC patients.The TME cell infiltration features were significantly in accordance with phenotypes of tumor immune-inflamed and immune desert in both patterns.The m6Ascore also served as an independent predictive factor in ACC patients.The somatic copy number variation(CNV)and patients prognosis can be predicted by m6A alteration patterns.Moreover,the ACC patients with high m6A scores had better overall survival(OS)and higher efficiency in immune checkpoint blockade therapy.Our work demonstrated the significance of m6A alteration to the ACC patients immunotherapy.The individual m6A alteration patterns analysis might contribute to ACC patients prognosis prediction and immunotherapy choice.
文摘The effect of a machine feeding rate(FR;1,1.5 and 2 Mg/h)and/or three levels of selected fineness degree(FD;3,5 and 7 mm)on machine performance,pellet physical parameters,required energy and production cost of three main types of broilers diets were examined in this experiment.The examined broiler diets were formulated to meet the Ross 308 strain requirements.A complete factorial design(3×3×3)was used to identify the effects of studied factors on the pellet mill machine and pellet production.The obtained results indicated that the pellet mill productivity significantly(p˂0.001)improved through increased pellet mill feeding rate level.In addition,the machine pelleting efficiency was found to be significantly affected by all studied variables and their interactions.While the total power consumption of the machine showed no variations under the impact of the tested factors or with any of their combinations.Regarding the pellet physical quality indices,all broiler diets with all selected FD and lower FR had the maximum durability and bulk density levels.Furthermore,lower feeding rates were associated with higher hardness degrees.The lowest production costs were substantially correlated with high FR and intermediate FD(5 mm).Furthermore,production costs were determined to be reduced in finisher broiler diets under different feeding rates.Moreover,manufacturing costs of finisher broiler meals were observed to decrease in several feeding rates.Overall,these findings indicate the capabilities of producing high-quality pellets and reducing the needed production costs by optimizing feeding rates to 2 Mg/h and 2 mm fineness in broiler diets.
