AIM： To investigate the single nucleotide polymorphisms （SNPs） in genes involved in bacterial recognition and the susceptibility to pouchitis or pouchitis severity. METHODS： Analyses of CD14 -260C〉T, CARD15/ NOD2...AIM： To investigate the single nucleotide polymorphisms （SNPs） in genes involved in bacterial recognition and the susceptibility to pouchitis or pouchitis severity. METHODS： Analyses of CD14 -260C〉T, CARD15/ NOD2 3020insC, Toll-like receptor （TLR）4 ＋896A〉G, TLR9 -1237T〉C, TLR9＋2848G〉A, and IRAKM ＋ 22148G〉A SNPs were performed in 157 ileal-pouch anal anastomosis （IPAA） patients （79 patients who did not develop pouchitis, 43 infrequent pouchitis patients, 35 chronic relapsing pouchitis patients） and 224 Italian Caucasian healthy controls. RESULTS： No significant differences were found in SNP frequencies between controls and IPAA patients. However, a significant difference in carriership frequency of the TLRg-1237C allele was found between the infrequent pouchitis and chronic relapsing pouchitis groups [P = 0.028, odd＇s ratio （OR） = 3.2, 95%CI = 1.2-8.6]. This allele uniquely represented a 4-locus TLR9 haplotype comprising both studied TLR9 SNPs in Caucasians. Carrier trait analysis revealed an enhanced combined carriership of the alleles TLR9 -1237C and CD14 -260T in the chronic relapsing pouchitis and infrequent pouchitis group （P = 0.018, OR = 4.1, 95%CI = 1.4 -12.3）. There is no evidence that the SNPs predispose to the need for IPAA surgery. The significant increase of the combined carriershoip of the CD14-260T and TLR9-1237C alleles in the chronic relapsing pouchitis group suggests that these markers identift a subgroup of IPAA patients with a rish of developing chronic or refractory pouchitis.展开更多
文摘AIM： To investigate the single nucleotide polymorphisms （SNPs） in genes involved in bacterial recognition and the susceptibility to pouchitis or pouchitis severity. METHODS： Analyses of CD14 -260C〉T, CARD15/ NOD2 3020insC, Toll-like receptor （TLR）4 ＋896A〉G, TLR9 -1237T〉C, TLR9＋2848G〉A, and IRAKM ＋ 22148G〉A SNPs were performed in 157 ileal-pouch anal anastomosis （IPAA） patients （79 patients who did not develop pouchitis, 43 infrequent pouchitis patients, 35 chronic relapsing pouchitis patients） and 224 Italian Caucasian healthy controls. RESULTS： No significant differences were found in SNP frequencies between controls and IPAA patients. However, a significant difference in carriership frequency of the TLRg-1237C allele was found between the infrequent pouchitis and chronic relapsing pouchitis groups [P = 0.028, odd＇s ratio （OR） = 3.2, 95%CI = 1.2-8.6]. This allele uniquely represented a 4-locus TLR9 haplotype comprising both studied TLR9 SNPs in Caucasians. Carrier trait analysis revealed an enhanced combined carriership of the alleles TLR9 -1237C and CD14 -260T in the chronic relapsing pouchitis and infrequent pouchitis group （P = 0.018, OR = 4.1, 95%CI = 1.4 -12.3）. There is no evidence that the SNPs predispose to the need for IPAA surgery. The significant increase of the combined carriershoip of the CD14-260T and TLR9-1237C alleles in the chronic relapsing pouchitis group suggests that these markers identift a subgroup of IPAA patients with a rish of developing chronic or refractory pouchitis.
