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上海凡纳滨对虾源副溶血弧菌耐药性及其耐药基因检测分析 被引量:7
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作者 高晓华 安伟 张明辉 《南方农业学报》 CAS CSCD 北大核心 2021年第3期827-836,共10页
【目的】掌握上海地区养殖凡纳滨对虾源副溶血弧菌的耐药性及耐药基因携带情况,并明确耐药表型与耐药基因间的相关性,为科学防控凡纳滨对虾副溶血弧菌病及揭示耐药机制提供理论依据。【方法】通过K-B纸片扩散法检测21株上海凡纳滨对虾... 【目的】掌握上海地区养殖凡纳滨对虾源副溶血弧菌的耐药性及耐药基因携带情况,并明确耐药表型与耐药基因间的相关性,为科学防控凡纳滨对虾副溶血弧菌病及揭示耐药机制提供理论依据。【方法】通过K-B纸片扩散法检测21株上海凡纳滨对虾源副溶血弧菌对14种常见抗生素的敏感性,采用二倍稀释法测定高度敏感抗生素对副溶血弧菌的最小抑菌浓度(MIC)和最小杀菌浓度(MBC),并以PCR检测其耐药基因的携带情况,包括β-内酰胺类耐药基因blaTEM和blaCARB、磺胺类耐药基因Sul Ⅱ和Sul Ⅲ、氨基糖苷类耐药基因strA和strB、四环素类耐药基因tetA及Ⅰ类整合子inT1基因。【结果】21株上海凡纳滨对虾源副溶血弧菌对14种常见抗生素表现出不同程度的多重耐药性,六重耐药1株(4.76%),五重耐药3株(14.29%),四重耐药5株(23.81%),三重耐药8株(38.10%),二重耐药4株(19.05%);AMP/PG/SMX和PG/SMX为优势耐药菌谱。上海凡纳滨对虾源副溶血弧菌对恩诺沙星的敏感率最高(90.48%),对应的MIC为0.10~1.60μg/mL、MBC为3.20~12.80μg/mL。blaTEM、blaCARB、Sul Ⅱ、Sul Ⅲ、strB和inT1等耐药基因的检出率分别为23.81%、71.43%、33.33%、19.05%、9.52%和23.81%,未检测出strA和tetA基因。从21株上海凡纳滨对虾源副溶血弧菌中共检出11种耐药基因型,其优势耐药基因型有blaCARB(28.57%)、blaCARB-blaTEM(14.29%)、blaCARB-Sul Ⅱ(14.29%)和Sul Ⅲ-inT1(9.52%)。除strA基因和tetA基因外,其余耐药基因与其对应抗生素耐药表型间均存在相关性,但其相关性不显著(P>0.05)。【结论】上海凡纳滨对虾源副溶血弧菌对14种常见抗生素表现出不同程度的敏感性,对恩诺沙星的敏感性最高,故可考虑将恩诺沙星作为上海地区凡纳滨对虾副溶血弧菌病防控的备选药物。此外,针对对虾源副溶血弧菌日趋严重的多重耐药问题,应同时加强副溶血弧菌耐药基因及整合子携带情况的监测,掌握其流行趋势,以提高对虾副溶血弧菌病的防控策略。 展开更多
关键词 凡纳滨对虾 副溶血弧菌 耐药性 耐药基因 上海
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SEISMIC RANDOM VIBRATION ANALYSIS OF LOCALLY NONLINEAR STRUCTURES 被引量:1
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作者 ZhaoYan LinJiahao +1 位作者 ZhangYahui anwei 《Acta Mechanica Solida Sinica》 SCIE EI 2003年第3期240-244,共5页
A nonlinear seismic analysis method for complex frame structures subjected to sta- tionary random ground excitations is proposed.The nonlinear elasto-plastic behaviors may take place only on a small part of the struct... A nonlinear seismic analysis method for complex frame structures subjected to sta- tionary random ground excitations is proposed.The nonlinear elasto-plastic behaviors may take place only on a small part of the structure.The Bouc-Wen differential equation model is used to model the hysteretic characteristics of the nonlinear components.The Pseudo Excitation Method (PEM)is used in solving the linearized random differential equations to replace the solution of the less efficient Lyapunov equation.Numerical results of a real bridge show that the method proposed is effective for practical engineering analysis. 展开更多
关键词 hysteretic system random vibration power spectral pseudo excitation method statistic linearization
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Determination of trophic relationships within a Bohai Bay food web using stable δ^(15)N and δ^(13)C analysis 被引量:12
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作者 WANYi HUJianying +5 位作者 ANLihui anwei YANGMin ItohMitsuaki HattoriTatsuya1 TAOShu 《Chinese Science Bulletin》 SCIE EI CAS 2005年第10期1021-1025,共5页
This study measured stable carbon and nitro- gen isotope ratios in phytoplankton, zooplankton, five inver- tebrates species, eight fishes species and three seabirds spe- cies collected in Bohai Bay. δ 13C ranged from... This study measured stable carbon and nitro- gen isotope ratios in phytoplankton, zooplankton, five inver- tebrates species, eight fishes species and three seabirds spe- cies collected in Bohai Bay. δ 13C ranged from ?25.38‰ to ?11.08‰ showing a relative low enrichment in the food web from Bohai Bay. The mean δ 13C of mullet is higher than that of other organisms, and this might be due to that mullet is migration fish and feeds mainly on inshore sources. δ 15N ranged from 4.08‰ to 13.98‰, and showed a step-wise en- richment with trophic level of 3.8‰. The δ 15N enrichment factor was used to construct an isotopic food web model to establish trophic relationships within this marine food web. According to this model, exact trophic levels of all organisms were estimated as 1.46?2.10, 1.91?3.32, 2.55?4.23 and 2.98?4.28 for plankton, invertebrates, fishes, and seabirds. 展开更多
关键词 渤海湾 营养网 营养水平 生态环境
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