In this work,we report innovative fiber optic biosensors exploiting localized surface plasmon resonance(LSPR)generated by gold nanostructures,specifically spherical nanoparticles(NPs)and nanostars(NSs),immobilized on ...In this work,we report innovative fiber optic biosensors exploiting localized surface plasmon resonance(LSPR)generated by gold nanostructures,specifically spherical nanoparticles(NPs)and nanostars(NSs),immobilized on uncladded silica multi-mode fibers to form a compact reflective probe architecture.The transition from NPs to NSs produced a redshift of the LSPR peak from 545 nm to 665 nm and markedly enhanced bulk refractive index sensitivity:16 nm diameter NPs reached 156 nm/RIU,while 78 nm diameter NSs achieved 402 nm/RIU.To demonstrate biosensing capability,the nanostructure coated fibers were biofunctionalized with anti-cortisol antibodies,enabling selective detection of cortisol,a key biomarker for stress and endocrine disorders.The resulting sensors exhibited a wide dynamic range(1 pg/mL100μg/mL),with the NS-based configuration achieving an outstanding limit of detection of 0.1 pg/mL.This broad range encompasses clinically relevant cortisol levels,underscoring the potential of LSPR-based fiber probes for non-invasive stress monitoring and personalized healthcare,where the synergy between nanostructure design and fiber integration paves the way for compact,label-free,highly sensitive point-of-care diagnostics.展开更多
基金European Commission(REFRESH CZ.10.03.01/00/22_003/0000048,NextGenEU PRIN 2022 CUP I53D23000390006)Funda????o para a Ciência e a Tecnologia(DigiAqua(PTDC/EEI EEE/0415/2021),CICECO UID/50011/2025)+2 种基金Ministry of Education,Youth,and Sports of the Czech Republic(VSB Technical University of Ostrava SP2026/028,SP2026/012)BRAZIL/CNPq/MCTI/FNDCT(22/2022)BRAZIL/FAPES(20/2022)。
文摘In this work,we report innovative fiber optic biosensors exploiting localized surface plasmon resonance(LSPR)generated by gold nanostructures,specifically spherical nanoparticles(NPs)and nanostars(NSs),immobilized on uncladded silica multi-mode fibers to form a compact reflective probe architecture.The transition from NPs to NSs produced a redshift of the LSPR peak from 545 nm to 665 nm and markedly enhanced bulk refractive index sensitivity:16 nm diameter NPs reached 156 nm/RIU,while 78 nm diameter NSs achieved 402 nm/RIU.To demonstrate biosensing capability,the nanostructure coated fibers were biofunctionalized with anti-cortisol antibodies,enabling selective detection of cortisol,a key biomarker for stress and endocrine disorders.The resulting sensors exhibited a wide dynamic range(1 pg/mL100μg/mL),with the NS-based configuration achieving an outstanding limit of detection of 0.1 pg/mL.This broad range encompasses clinically relevant cortisol levels,underscoring the potential of LSPR-based fiber probes for non-invasive stress monitoring and personalized healthcare,where the synergy between nanostructure design and fiber integration paves the way for compact,label-free,highly sensitive point-of-care diagnostics.
