The rosemary essential oil(REO)and oregano essential oil(OEO)are known for their inherent biomedical properties,which were further enhanced when converted into nanoformulation.The conversion of essential oils into nan...The rosemary essential oil(REO)and oregano essential oil(OEO)are known for their inherent biomedical properties,which were further enhanced when converted into nanoformulation.The conversion of essential oils into nanoformulation may improve their stability,bioavailability,and functionality.The FTIR spectra of nano-emulsions revealed peaks at 1080.14 cm^(-1),1629.85 cm^(-1),2115.91 cm^(-1),2926.01 cm^(-1) and 3196.05 cm^(-1) for rosemary nanoemulsion(RNE).Also,a peak shift was observed at 1627.92 cm^(-1) and 2341.58 cm^(-1) for oregano nanoemulsion(ONE)highlighting that the essential oils were stably encapsulated with the surfactant.The particle size analysis(PSA)revealed that the mean droplet sizes of RNE and ONE were 129.2 nm and 25.98 nm respectively which indicates the stability and uniform distribution of these nanoemulsions.The HR-TEM showed that the nanoemulsions were spherical,smooth,and well-dispersed with droplet sizes below 100 nm.The antioxidant capacities were assessed using FRAP,DPPH,and H2O_(2) scavenging tests,and the results indicated that RNE and ONE showed higher antioxidant capacity than REO and OEO.In the BSA denaturation assay,protein denaturation was significantly inhibited,indicating the strong anti-inflammatory properties of RNE and ONE.Similarly,both RNE and ONE showed a rise in antimicrobial efficacy against different microorganisms.The MTT cytotoxicity assay on AGS cell lines showed that the nanoemulsions were biocompatible and had improved anticancer activity like that of the standard.These findings show that encapsulating essential oils stabilizes and boosts their antimicrobial,antioxidant,anti-inflammatory and anti-cancer potential,thereby,making them more effective in various applications.展开更多
基金supported by Researchers Supporting Project No.(RSP2025R364),King Saud University,Riyadh,Saudi Arabia。
文摘The rosemary essential oil(REO)and oregano essential oil(OEO)are known for their inherent biomedical properties,which were further enhanced when converted into nanoformulation.The conversion of essential oils into nanoformulation may improve their stability,bioavailability,and functionality.The FTIR spectra of nano-emulsions revealed peaks at 1080.14 cm^(-1),1629.85 cm^(-1),2115.91 cm^(-1),2926.01 cm^(-1) and 3196.05 cm^(-1) for rosemary nanoemulsion(RNE).Also,a peak shift was observed at 1627.92 cm^(-1) and 2341.58 cm^(-1) for oregano nanoemulsion(ONE)highlighting that the essential oils were stably encapsulated with the surfactant.The particle size analysis(PSA)revealed that the mean droplet sizes of RNE and ONE were 129.2 nm and 25.98 nm respectively which indicates the stability and uniform distribution of these nanoemulsions.The HR-TEM showed that the nanoemulsions were spherical,smooth,and well-dispersed with droplet sizes below 100 nm.The antioxidant capacities were assessed using FRAP,DPPH,and H2O_(2) scavenging tests,and the results indicated that RNE and ONE showed higher antioxidant capacity than REO and OEO.In the BSA denaturation assay,protein denaturation was significantly inhibited,indicating the strong anti-inflammatory properties of RNE and ONE.Similarly,both RNE and ONE showed a rise in antimicrobial efficacy against different microorganisms.The MTT cytotoxicity assay on AGS cell lines showed that the nanoemulsions were biocompatible and had improved anticancer activity like that of the standard.These findings show that encapsulating essential oils stabilizes and boosts their antimicrobial,antioxidant,anti-inflammatory and anti-cancer potential,thereby,making them more effective in various applications.
