Three dimensional TiO2 scaffolds are receiving renewed attention for bone tissue engineering (TE) due totheir biocompatibility and attractive mechanical properties. However the bioactivity of these scaffolds iscompara...Three dimensional TiO2 scaffolds are receiving renewed attention for bone tissue engineering (TE) due totheir biocompatibility and attractive mechanical properties. However the bioactivity of these scaffolds iscomparatively lower than that of bioactive glass or hydroxyapatite (HA) scaffolds. One strategy toimprove bioactivity is to functionalize the surface of the scaffolds using biomolecules. Alkaline phosphatase(ALP) was chosen in this study due to its important role in the bone mineralization process. Thecurrent study investigated the ALP functionalization of 3D titanium dioxide scaffolds using selfpolymerizationof dopamine. Robust titanium scaffolds (compressive strength^2.7 ± 0.3 MPa) wereproduced via foam replica method. Enzyme grafting was performed by dip-coating in polydopamine/ALPsolution. The presence of ALP was indirectly confirmed by contact angle measurements and enzymaticactivity study. The influence of the enzyme on the bioactivity, e.g. hydroxyapatite formation on thescaffold surface, was measured in simulated body fluid (SBF). After 28 days in SBF, 5 mg ALP coatedtitania scaffolds exhibited increased hydroxyapatite formation. It was thus confirmed that ALP enhancesthe bioactivity of titania scaffolds, converting an inert bioceramic in an attractive bioactive system forbone TE.展开更多
基金The authors would like to acknowledge Dr.Tobias Fey(University of Erlangen-Nuremberg)for helping with XRD measurementsMr.Marvin Valley(Institute of Biomaterials,University of Erlangen-Nuremberg)for experimental support.
文摘Three dimensional TiO2 scaffolds are receiving renewed attention for bone tissue engineering (TE) due totheir biocompatibility and attractive mechanical properties. However the bioactivity of these scaffolds iscomparatively lower than that of bioactive glass or hydroxyapatite (HA) scaffolds. One strategy toimprove bioactivity is to functionalize the surface of the scaffolds using biomolecules. Alkaline phosphatase(ALP) was chosen in this study due to its important role in the bone mineralization process. Thecurrent study investigated the ALP functionalization of 3D titanium dioxide scaffolds using selfpolymerizationof dopamine. Robust titanium scaffolds (compressive strength^2.7 ± 0.3 MPa) wereproduced via foam replica method. Enzyme grafting was performed by dip-coating in polydopamine/ALPsolution. The presence of ALP was indirectly confirmed by contact angle measurements and enzymaticactivity study. The influence of the enzyme on the bioactivity, e.g. hydroxyapatite formation on thescaffold surface, was measured in simulated body fluid (SBF). After 28 days in SBF, 5 mg ALP coatedtitania scaffolds exhibited increased hydroxyapatite formation. It was thus confirmed that ALP enhancesthe bioactivity of titania scaffolds, converting an inert bioceramic in an attractive bioactive system forbone TE.
