Carbamates insecticides are used increasingly in organochlorine insecticides for the control of insect pests. It agriculture as a replacement for environmentally more persistent was the most used pesticides in Loukkou...Carbamates insecticides are used increasingly in organochlorine insecticides for the control of insect pests. It agriculture as a replacement for environmentally more persistent was the most used pesticides in Loukkous-Morocco. Conventional methods employed to detect/analyze carbamates residues are time consuming and require sophisticated equipment only available in well-equipped laboratories. In addition, the conventional methods usually require a lot of complex pre-treatment of samples. Therefore, convenient and rapid pesticide detection system is urgently needed. In this order, we aimed to develop a rapid ELISA （enzyme-linked immuno-Sorbent Assay） for detection of some carbamates such as carbendazim [methyl 2-benzimidazole carbamate] and carbofuran [2,3-dihydro-2,2-dimethyl-7-benzofuranylmethyl carbamate]. To develop an immunoassay for carbendazim and carbofuran we have synthesized molecules with acid function （haptens with 4 and 5 carbons） that are coupled with BSA protein and injected to the rabbits, collected antibodies are used for the achievements of the immunoanalytical assay.展开更多
文摘Carbamates insecticides are used increasingly in organochlorine insecticides for the control of insect pests. It agriculture as a replacement for environmentally more persistent was the most used pesticides in Loukkous-Morocco. Conventional methods employed to detect/analyze carbamates residues are time consuming and require sophisticated equipment only available in well-equipped laboratories. In addition, the conventional methods usually require a lot of complex pre-treatment of samples. Therefore, convenient and rapid pesticide detection system is urgently needed. In this order, we aimed to develop a rapid ELISA （enzyme-linked immuno-Sorbent Assay） for detection of some carbamates such as carbendazim [methyl 2-benzimidazole carbamate] and carbofuran [2,3-dihydro-2,2-dimethyl-7-benzofuranylmethyl carbamate]. To develop an immunoassay for carbendazim and carbofuran we have synthesized molecules with acid function （haptens with 4 and 5 carbons） that are coupled with BSA protein and injected to the rabbits, collected antibodies are used for the achievements of the immunoanalytical assay.
