使用质粒DNA评价法(Plasmid DNA assay)研究了在北京市市区、郊区卫星城市南口镇和清洁对照点十三陵水库地区采集的PM10和PM2.5样品及部分源颗粒物的生物活性.结果表明,沙尘暴期间采集的颗粒物对DNA的生物活性小于非沙尘暴期间采集的样...使用质粒DNA评价法(Plasmid DNA assay)研究了在北京市市区、郊区卫星城市南口镇和清洁对照点十三陵水库地区采集的PM10和PM2.5样品及部分源颗粒物的生物活性.结果表明,沙尘暴期间采集的颗粒物对DNA的生物活性小于非沙尘暴期间采集的样品.不同时间和地点采集的大气颗粒物样品对质粒DNA的损伤差别很大,它们的TM50(造成50％的DNA损伤所需的剂量)相差可高达一个数量级,其原因,可能是北京市大气颗粒物中矿物质量百分比的变化引起了生物活性较强的细颗粒物相对含量的改变.同PM10相比,大部分PM2.5样品的生物活性相对较大.最后,对比水溶和总体颗粒物质粒DNA评价的结果得出,大气颗粒物中的水溶组分是其生物活性的主要来源.展开更多
Plasmid DNA assay is a newly-developed in vitro method to investigate bioreactivity of particles. In this paper, this method was used to study the bioreactivity of PM10 (particulate matter with aerodynamic diameter of...Plasmid DNA assay is a newly-developed in vitro method to investigate bioreactivity of particles. In this paper, this method was used to study the bioreactivity of PM10 (particulate matter with aerodynamic diameter of less than 10 mm) and PM2.5 (particulate matter with aerodynamic diameter of less than 2.5 mm). Samples and dust storm particles were collected in 2001 in an urban area, a satellite city and a clean air area in Beijing. A big difference was found for oxidative DNA stress induced by different particulate matter (PM) samples, with the TM50 (particle mass causing 50% damage to DNA) values varying by a factor over 10. This was closely dependent on the sizes of particles as well as the variation in relative proportion of mineral matter. PM2.5 samples generally impose larger oxidative stress on plasmid DNA than PM10 samples. Airborne particles collected during dust storm episodes, usually with a higher proportion of mineral matter, have a much lower oxidative capacity than those collected during non-dust storm episodes. PM samples and their water-soluble fractions usually have similar bioreactivities, demonstrating that oxidative capacity of Beijing airborne particles is mainly sourced from their water-soluble fractions.展开更多
文摘使用质粒DNA评价法(Plasmid DNA assay)研究了在北京市市区、郊区卫星城市南口镇和清洁对照点十三陵水库地区采集的PM10和PM2.5样品及部分源颗粒物的生物活性.结果表明,沙尘暴期间采集的颗粒物对DNA的生物活性小于非沙尘暴期间采集的样品.不同时间和地点采集的大气颗粒物样品对质粒DNA的损伤差别很大,它们的TM50(造成50％的DNA损伤所需的剂量)相差可高达一个数量级,其原因,可能是北京市大气颗粒物中矿物质量百分比的变化引起了生物活性较强的细颗粒物相对含量的改变.同PM10相比,大部分PM2.5样品的生物活性相对较大.最后,对比水溶和总体颗粒物质粒DNA评价的结果得出,大气颗粒物中的水溶组分是其生物活性的主要来源.
文摘Plasmid DNA assay is a newly-developed in vitro method to investigate bioreactivity of particles. In this paper, this method was used to study the bioreactivity of PM10 (particulate matter with aerodynamic diameter of less than 10 mm) and PM2.5 (particulate matter with aerodynamic diameter of less than 2.5 mm). Samples and dust storm particles were collected in 2001 in an urban area, a satellite city and a clean air area in Beijing. A big difference was found for oxidative DNA stress induced by different particulate matter (PM) samples, with the TM50 (particle mass causing 50% damage to DNA) values varying by a factor over 10. This was closely dependent on the sizes of particles as well as the variation in relative proportion of mineral matter. PM2.5 samples generally impose larger oxidative stress on plasmid DNA than PM10 samples. Airborne particles collected during dust storm episodes, usually with a higher proportion of mineral matter, have a much lower oxidative capacity than those collected during non-dust storm episodes. PM samples and their water-soluble fractions usually have similar bioreactivities, demonstrating that oxidative capacity of Beijing airborne particles is mainly sourced from their water-soluble fractions.
