AIM To explore the expression and changes of hepatoma specific alkaline phospatase (ALP) in rats during canceration.METHODS The ALPs and isoenzymes of rat livers and sera were investigated in SD hepatomas induced with...AIM To explore the expression and changes of hepatoma specific alkaline phospatase (ALP) in rats during canceration.METHODS The ALPs and isoenzymes of rat livers and sera were investigated in SD hepatomas induced with 0.05% 2-fluoenylacetamide (2-FAA).RESULTS By pathological examination and biochemical analysis. ALPs were overexpressed in rat livers during canceration and then were secreted into blood. Serum total ALP activities, liver ALP specific activities (U/g) including soluble and membrane-combined ALP activities of each group were all significantly higher (P＜0.01) than those of control group. The average ratios of soluble ALP to membrane-combined ALP were increased significantly after 6 weeks. ALP isoenzymes of rat sera and livers showed 5 bands on PAGE: ALP-Ⅰ and ALP-Ⅱ were specific for normal liver and rat hepatoma tissues, the ALP-Ⅱ appeared in rat liver after 6 weeks and in sera after 8 weeks.CONCLUSIONS ALP with carcino-embryonic protein was overexpressed in hepatoma tissues; the abnormal ALP-Ⅱ of ALP isoenzymes in sera and liver of rats can be used as a tumor marker for early diagnosis of rat hepatoma.展开更多
文摘AIM To explore the expression and changes of hepatoma specific alkaline phospatase (ALP) in rats during canceration.METHODS The ALPs and isoenzymes of rat livers and sera were investigated in SD hepatomas induced with 0.05% 2-fluoenylacetamide (2-FAA).RESULTS By pathological examination and biochemical analysis. ALPs were overexpressed in rat livers during canceration and then were secreted into blood. Serum total ALP activities, liver ALP specific activities (U/g) including soluble and membrane-combined ALP activities of each group were all significantly higher (P＜0.01) than those of control group. The average ratios of soluble ALP to membrane-combined ALP were increased significantly after 6 weeks. ALP isoenzymes of rat sera and livers showed 5 bands on PAGE: ALP-Ⅰ and ALP-Ⅱ were specific for normal liver and rat hepatoma tissues, the ALP-Ⅱ appeared in rat liver after 6 weeks and in sera after 8 weeks.CONCLUSIONS ALP with carcino-embryonic protein was overexpressed in hepatoma tissues; the abnormal ALP-Ⅱ of ALP isoenzymes in sera and liver of rats can be used as a tumor marker for early diagnosis of rat hepatoma.
