An important interface modification for capillary isoelectric focusing mass spectrometry(CIEF MS) is reported. A capillary needle with a sheath flow tube pre mounted in one device serves as both the CIEF cathodic end ...An important interface modification for capillary isoelectric focusing mass spectrometry(CIEF MS) is reported. A capillary needle with a sheath flow tube pre mounted in one device serves as both the CIEF cathodic end and the ESI interface. The needle was inserted into the cathode reservoir at the focusing stage, and then removed to the front of sampler orifice of MS at the detection stage. Therefore, the shut off of the high voltage is not necessary and a better focusing protein zone is maintained during the ESI stage. The optimization of the experimental condition has been investigated and the two dimensional image of the E. coli proteins has been obtained, which is comparable with that from a 2D gel.展开更多
The studies of SCAIF I(an unknown protein) by HPLC and ESIMS was reported in this paper. The SCAIF I is a kind of antiangiogenesis extracted from shark cartilage. The extracted protein was purified by HPLC and then st...The studies of SCAIF I(an unknown protein) by HPLC and ESIMS was reported in this paper. The SCAIF I is a kind of antiangiogenesis extracted from shark cartilage. The extracted protein was purified by HPLC and then studied by ESIMS. The molecule weight of the SCAIF I protein was confirmed to be 15 601 in the first time. Peptide map of the unknown protein was obtained by HPLC ESIMS with a trypsin digestion and possible partial sequences were deduced with the online CID technique. With partial sequence information the homologous proteins are searched with protein database. The homology of SCAIF I summarized in this work is useful in further studies of this unknown protein.展开更多
文摘An important interface modification for capillary isoelectric focusing mass spectrometry(CIEF MS) is reported. A capillary needle with a sheath flow tube pre mounted in one device serves as both the CIEF cathodic end and the ESI interface. The needle was inserted into the cathode reservoir at the focusing stage, and then removed to the front of sampler orifice of MS at the detection stage. Therefore, the shut off of the high voltage is not necessary and a better focusing protein zone is maintained during the ESI stage. The optimization of the experimental condition has been investigated and the two dimensional image of the E. coli proteins has been obtained, which is comparable with that from a 2D gel.
文摘The studies of SCAIF I(an unknown protein) by HPLC and ESIMS was reported in this paper. The SCAIF I is a kind of antiangiogenesis extracted from shark cartilage. The extracted protein was purified by HPLC and then studied by ESIMS. The molecule weight of the SCAIF I protein was confirmed to be 15 601 in the first time. Peptide map of the unknown protein was obtained by HPLC ESIMS with a trypsin digestion and possible partial sequences were deduced with the online CID technique. With partial sequence information the homologous proteins are searched with protein database. The homology of SCAIF I summarized in this work is useful in further studies of this unknown protein.
