目的探讨不同剂量甲氨蝶呤对急性淋巴细胞白血病患儿CD4^(+)、CD8^(+)百分比、CD4^(+)/CD8^(+)比值的影响,为临床治疗小儿急性淋巴细胞白血病提供参考。方法选取2017年4月至2020年4月绵阳市中心医院收治的90例急性淋巴细胞白血病患儿作...目的探讨不同剂量甲氨蝶呤对急性淋巴细胞白血病患儿CD4^(+)、CD8^(+)百分比、CD4^(+)/CD8^(+)比值的影响,为临床治疗小儿急性淋巴细胞白血病提供参考。方法选取2017年4月至2020年4月绵阳市中心医院收治的90例急性淋巴细胞白血病患儿作为研究对象,所有患儿均经过诱导缓解化疗和早期强化化疗,按照使用甲氨蝶呤剂量不同分为A组(3 g/m^(2))与B组(5 g/m2),各45例。比较用药后不同时间点两组患儿甲氨蝶呤血药浓度与消除速率,不同级别患儿四氢叶酸钙解救次数和解救总剂量,化疗前、化疗后3个月两组患儿CD4^(+)、CD8^(+)百分比、CD4^(+)/CD8^(+)比值,以及化疗期间不良反应发生情况。结果与用药后30 min比,用药后12~72 h两组患儿甲氨蝶呤血药浓度呈降低趋势,且用药后各时间点B组高于A组;与用药后12 h比,用药后24~72 h A组患儿甲氨蝶呤消除速率呈先升高后降低趋势,B组患儿甲氨蝶呤消除速率呈逐渐降低趋势,且用药后各时间点B组高于A组;B组C48 h 1~3级、C48 h 4~6级患儿四氢叶酸钙解救次数、解救总剂量高于A组;化疗后3个月两组患儿CD4^(+)百分比、CD4^(+)/CD8^(+)比值均高于化疗前,且B组高于A组,而CD8^(+)百分比均低于化疗前,且B组低于A组;治疗期间B组患儿各不良反应发生率均高于A组(均P<0.05)。结论大剂量甲氨蝶呤可增加急性淋巴细胞白血病患儿血药浓度,同时提高四氢叶酸钙用药时间和剂量,改善免疫功能,临床可对不良反应及时进行对症处理,以改善患儿预后。展开更多
[Objective] This study aimed to construct the full-length cDNA library for ger- minating seeds of Phyllostachys heterocycla [Method] Germinating seeds of P. hetero- cycla were used as experimental materials to constru...[Objective] This study aimed to construct the full-length cDNA library for ger- minating seeds of Phyllostachys heterocycla [Method] Germinating seeds of P. hetero- cycla were used as experimental materials to construct the full-length cDNA library by using Oligo-capping method. [Result] The constructed library has a total capacity of 6.5×10^6 recombinant clones, and a low proportion of clones without inserted frag- ments; the size of inserted fragments ranges between 0.3-5.0 kb, with strict classifi- cation and ideal consistency. Furthermore, the proportion of clones harboring long in- serted fragments （1.0-5.0 kb） is as high as 30%, achieving the standard for high- quality full-length cDNA library. [Conclusion] The full-length cDNA library of germinat- ing seeds of P. heterocycla was successfully constructed, which laid important foun- dation for the functional genomics research of bamboo plants.展开更多
rDNA internal transcribed spacer analysis(rDNA ITS)was used to study diversity of 12 representative mycorrhizal Rhizoctonia strains,which were isolated from Chinese orchids(Cymbidium)distributed in different sites and...rDNA internal transcribed spacer analysis(rDNA ITS)was used to study diversity of 12 representative mycorrhizal Rhizoctonia strains,which were isolated from Chinese orchids(Cymbidium)distributed in different sites and belonged to different ecologic types.The Blast results indicated that all these strains belonged to Epulorhiza or Tulasnella,which was fully consistent with identification as Epulorhiza with morphological method.Additionally,based on rDNA ITS sequence cluster analysis,dendrogram of Cymbidium mycorrhizal strains showed that the distributed environments and orchids species were two crucial factors that affected the specificity-relation between Chinese orchids(Cymbidium)symbiotic mycorrhizae and hosts.展开更多
文摘目的探讨不同剂量甲氨蝶呤对急性淋巴细胞白血病患儿CD4^(+)、CD8^(+)百分比、CD4^(+)/CD8^(+)比值的影响,为临床治疗小儿急性淋巴细胞白血病提供参考。方法选取2017年4月至2020年4月绵阳市中心医院收治的90例急性淋巴细胞白血病患儿作为研究对象,所有患儿均经过诱导缓解化疗和早期强化化疗,按照使用甲氨蝶呤剂量不同分为A组(3 g/m^(2))与B组(5 g/m2),各45例。比较用药后不同时间点两组患儿甲氨蝶呤血药浓度与消除速率,不同级别患儿四氢叶酸钙解救次数和解救总剂量,化疗前、化疗后3个月两组患儿CD4^(+)、CD8^(+)百分比、CD4^(+)/CD8^(+)比值,以及化疗期间不良反应发生情况。结果与用药后30 min比,用药后12~72 h两组患儿甲氨蝶呤血药浓度呈降低趋势,且用药后各时间点B组高于A组;与用药后12 h比,用药后24~72 h A组患儿甲氨蝶呤消除速率呈先升高后降低趋势,B组患儿甲氨蝶呤消除速率呈逐渐降低趋势,且用药后各时间点B组高于A组;B组C48 h 1~3级、C48 h 4~6级患儿四氢叶酸钙解救次数、解救总剂量高于A组;化疗后3个月两组患儿CD4^(+)百分比、CD4^(+)/CD8^(+)比值均高于化疗前,且B组高于A组,而CD8^(+)百分比均低于化疗前,且B组低于A组;治疗期间B组患儿各不良反应发生率均高于A组(均P<0.05)。结论大剂量甲氨蝶呤可增加急性淋巴细胞白血病患儿血药浓度,同时提高四氢叶酸钙用药时间和剂量,改善免疫功能,临床可对不良反应及时进行对症处理,以改善患儿预后。
基金Supported by Specialized Fund for the Basic Research Operating Expenses Program of International Centre for Bamboo and Rattan(163201300812618-7)Special Fund for Research and Development of Forestry Nonprofit Industry(200704001)~~
文摘[Objective] This study aimed to construct the full-length cDNA library for ger- minating seeds of Phyllostachys heterocycla [Method] Germinating seeds of P. hetero- cycla were used as experimental materials to construct the full-length cDNA library by using Oligo-capping method. [Result] The constructed library has a total capacity of 6.5×10^6 recombinant clones, and a low proportion of clones without inserted frag- ments; the size of inserted fragments ranges between 0.3-5.0 kb, with strict classifi- cation and ideal consistency. Furthermore, the proportion of clones harboring long in- serted fragments （1.0-5.0 kb） is as high as 30%, achieving the standard for high- quality full-length cDNA library. [Conclusion] The full-length cDNA library of germinat- ing seeds of P. heterocycla was successfully constructed, which laid important foun- dation for the functional genomics research of bamboo plants.
文摘rDNA internal transcribed spacer analysis(rDNA ITS)was used to study diversity of 12 representative mycorrhizal Rhizoctonia strains,which were isolated from Chinese orchids(Cymbidium)distributed in different sites and belonged to different ecologic types.The Blast results indicated that all these strains belonged to Epulorhiza or Tulasnella,which was fully consistent with identification as Epulorhiza with morphological method.Additionally,based on rDNA ITS sequence cluster analysis,dendrogram of Cymbidium mycorrhizal strains showed that the distributed environments and orchids species were two crucial factors that affected the specificity-relation between Chinese orchids(Cymbidium)symbiotic mycorrhizae and hosts.
