Based on temperature data of meteorological stations from 1971 to 2008 in Tibet,the temporal and spatial variation of maximum andminimum temperature in Tibet was analyzed.The results showed that both maximum temperatu...Based on temperature data of meteorological stations from 1971 to 2008 in Tibet,the temporal and spatial variation of maximum andminimum temperature in Tibet was analyzed.The results showed that both maximum temperature andminimum temperature increased distinctly,the warming amplitude of winter was the highest among the four seasons,and next came spring.The increment ofminimum temperature was visibly over that of maximum temperature,particularlyminimum temperature in winter with significant increment.For spatial variation,maximum temperature in most stations increased except particular stations,while theminimum temperature in all stations rose.In addition,the space variation law ofminimum temperature,being more obvious thanminimum temperature,increased from southeast to northwest with different spatial changes in various seasons.From decadal variation,both maximum andminimum temperature appeared increase from 1970s to the first eight years in the 21st century,and the rise ofminimum temperature was significant greater than maximum temperature.The increase of maximum andminimum temperature was the highest from 2001 to 2008,whereas the lowest in 1970s.展开更多
采用梯度压力驯化法从高海拔地区土壤样品中分离到1株能以BDE-99作为碳源生长的细菌菌株,命名为BHS-4,通过生理生化及对16S r DNA扩增,T/A克隆后测序鉴定,并进一步分析初始pH、温度、碳源、细胞表胞疏水性(MATH法)及共存重金属离子对菌...采用梯度压力驯化法从高海拔地区土壤样品中分离到1株能以BDE-99作为碳源生长的细菌菌株,命名为BHS-4,通过生理生化及对16S r DNA扩增,T/A克隆后测序鉴定,并进一步分析初始pH、温度、碳源、细胞表胞疏水性(MATH法)及共存重金属离子对菌株降解效果的影响。结果表明:菌株BHS-4与产碱杆菌属Alcaligenes cupidus同源性为99%。菌株降解BDE-99最适pH和温度分别为8.0、20℃,经11 d培养后,对浓度0.4 mg·L^(-1)的BDE-99,降解率达86.1%,在15~20℃范围具有较高降解率。降解特性研究结果显示,乳糖、麦芽糖和葡萄糖的添加促进了BHS-4对BDE-99的降解,而淀粉和乙酸钠添加对降解率产生了显著抑制作用(P<0.05)。NH_4NO_3的添加,使细胞表面疏水率(CSH)增加了49.5%,CSH与降解率呈极显著正相关(r=0.99,P<0.01)。Cu^(2+)、Zn^(2+)在0.1~0.3 mg·L^(-1)浓度下促进BDE-99降解;Cr^(6+)在0.1~0.6 mg·L^(-1)范围内对降解作用影响表现为先抑制、再促进、最后抑制。菌株BHS-4能在较低温度下对BDE-99进行高效降解且对Cu^(2+)、Zn^(2+)、Cr^(6+)具有一定的耐性,可应用于低温地区BDE-99的生物处理,同时可为进一步研究低温条件下BDE-99生物降解的代谢途径和机理提供一定的基础资料。展开更多
文摘Based on temperature data of meteorological stations from 1971 to 2008 in Tibet,the temporal and spatial variation of maximum andminimum temperature in Tibet was analyzed.The results showed that both maximum temperature andminimum temperature increased distinctly,the warming amplitude of winter was the highest among the four seasons,and next came spring.The increment ofminimum temperature was visibly over that of maximum temperature,particularlyminimum temperature in winter with significant increment.For spatial variation,maximum temperature in most stations increased except particular stations,while theminimum temperature in all stations rose.In addition,the space variation law ofminimum temperature,being more obvious thanminimum temperature,increased from southeast to northwest with different spatial changes in various seasons.From decadal variation,both maximum andminimum temperature appeared increase from 1970s to the first eight years in the 21st century,and the rise ofminimum temperature was significant greater than maximum temperature.The increase of maximum andminimum temperature was the highest from 2001 to 2008,whereas the lowest in 1970s.
