目的:探讨有氧运动对不同年龄小鼠海马哺乳动物雷帕霉素靶蛋白(mammalian target of rapamycin,mTOR)信号通路活性的影响及其与认知功能的关系。方法:将1月龄C57BL/6雄性小鼠90只,随机分为有氧运动(E)组(n=45)和安静对照(C)组(n=45),有...目的:探讨有氧运动对不同年龄小鼠海马哺乳动物雷帕霉素靶蛋白(mammalian target of rapamycin,mTOR)信号通路活性的影响及其与认知功能的关系。方法:将1月龄C57BL/6雄性小鼠90只,随机分为有氧运动(E)组(n=45)和安静对照(C)组(n=45),有氧运动组小鼠进行75%VO2max强度的有氧跑台运动干预;两组分别至小鼠3、12、20月龄时,行Morris水迷宫实验测试认知功能后,处死取材(n=15);采用Western blot法检测小鼠海马mTOR信号通路蛋白mTOR、mTOR丝氨酸2448位点磷酸化[p-mTOR(Ser2448)]、核糖体S6蛋白激酶1苏氨酸389位点磷酸化[p-S6K1(Thr389)]、真核生物翻译起始因子4E结合蛋白丝氨酸65位点磷酸化[p-4EBP1(Ser65)]及其上游脑源性神经营养因子(brainderived neurotrophic factor,BDNF)表达水平;采用免疫组织化学染色法检测小鼠海马BDNF蛋白阳性细胞并计算积分光密度。结果:水迷宫定位航行试验第3天开始,12月龄和20月龄小鼠逃避潜伏期显著长于3月龄小鼠(P<0.05,P<0.01),且20月龄较12月龄逃避潜伏期更长(P<0.05,P<0.01);目标象限航程和原平台位置穿越次数,12月龄小鼠显著低于3月龄小鼠(P<0.05),20月龄小鼠目标象限航程显著低于3、12月龄小鼠(P<0.01)。与同龄对照组相比,运动组逃避潜伏期显著缩短(P<0.05),目标象限航程显著增加(P<0.01)。20月龄小鼠p-mTOR(Ser2448)、p-S6K1(Thr389)、p-4EBP1(Ser65)及BDNF表达水平显著低于3月龄或12月龄小鼠(P<0.05,P<0.01);对于3月龄小鼠海马p-mTOR(Ser2448)、p-S6K1(Thr389)、p-4EBP1(Ser65)表达水平,运动组显著高于对照组(P<0.01);对于12和20月龄小鼠p-mTOR(Ser2448)、p-S6K1(Thr389)、p-4EBP1(Ser65)表达水平,运动组显著低于同龄对照组(P<0.05,P<0.01);与同龄对照组相比,运动组小鼠海马BDNF的表达水平显著提高(P<0.01)。结论:有氧运动促进幼年小鼠认知功能发育并延缓老年小鼠认知功能增龄性退变,这一作用效果可能通过激活幼年小鼠海马mTOR信号通路或抑制成年后该信号通路活性而实现;且提示mTOR信号通路活性对发育期小鼠海马记忆的必要性,但其过度活跃对老年小鼠认知可能产生不利影响。展开更多
[Objective] The aim was to study the antioxidant effect of polyphenols from pomegranate peel in vivo. [Method] The Kunming rats were randomly divided into a control group, a low-dose group, a middle-dose group and a h...[Objective] The aim was to study the antioxidant effect of polyphenols from pomegranate peel in vivo. [Method] The Kunming rats were randomly divided into a control group, a low-dose group, a middle-dose group and a high-dose group,n=10; the protein content, the activities of the superoxide dismutase（SOD） and the glutathione peroxidase（GSH-PX）, the content of the maleic dialdehyde（MDA） in serum and liver tissue of the rats from different groups were determined. [Result]The polyphenols of pomegranate peel could increase protein content, activities of superoxide dismutase（SOD） and glutathione peroxidase（GSH-PX） in serum and liver tissue, and decrease the maleic dialdehyde（MDA） content simultaneously. [Conclusion] Polyphenols of pomegranate peel have strong antioxidant activity in vivo.展开更多
基金Supported by Scientific Research Projects Units of Science and Technology Department of Xinjiang Uygur Autonomous Region for Financial Support(Project No.2013911072)~~
文摘[Objective] The aim was to study the antioxidant effect of polyphenols from pomegranate peel in vivo. [Method] The Kunming rats were randomly divided into a control group, a low-dose group, a middle-dose group and a high-dose group,n=10; the protein content, the activities of the superoxide dismutase（SOD） and the glutathione peroxidase（GSH-PX）, the content of the maleic dialdehyde（MDA） in serum and liver tissue of the rats from different groups were determined. [Result]The polyphenols of pomegranate peel could increase protein content, activities of superoxide dismutase（SOD） and glutathione peroxidase（GSH-PX） in serum and liver tissue, and decrease the maleic dialdehyde（MDA） content simultaneously. [Conclusion] Polyphenols of pomegranate peel have strong antioxidant activity in vivo.
