摘要
目的 确定鼠疫耶尔森菌DNA标识序列 ,以用于鼠疫耶尔森菌的快速检测和鉴定。方法 通过芯片比较基因组杂交和PCR验证 ,鉴定鼠疫耶尔森菌DNA标识序列 ,针对标识序列设计特定的PCR引物。结果 鼠疫耶尔森菌基因组中存在 3个区段 ,共 2 8条基因 ,均是鼠疫耶尔森菌DNA标识序列。针对其中 3条基因设计PCR引物 ,能特异性扩增出鼠疫耶尔森菌 ,与来自其他非鼠疫耶尔森菌的DNA无交叉反应。结论 鼠疫耶尔森菌存在DNA标识序列 ,并能用于鼠疫耶尔森菌的快速检测与鉴定。
Objective To identify the DNA tag sequences with the purpose of rapid and specific characterization of Y. pestis. Methods DNA microarray hybridization combined with PCR was used to perform genomic comparison between strains of Y. pestis and Y. pseudotuberculosis in order to screen and identify Y. pestis-specific genes. Results Twenty eight signature genes of Y. pestis were discovered. Three pairs of Y. pestis-specific primers were designed according to tag genes and proved to amplify the specific sequences of the target bacterium, showing no cross-reaction with the closely related Y. pseudotuberculosis and a large collection of genomic DNAs from other organisms. Conclusion DNA tag sequence is an ideal target for the rapid detection and identification of Y. pestis by PCR method.
出处
《解放军医学杂志》
CAS
CSCD
北大核心
2004年第4期307-309,共3页
Medical Journal of Chinese People's Liberation Army
基金
国家"8 63"基金 (编号 2 0 0 1AA2 2 30 61 )
国家自然科学基金 (编号30 371 2 84)资助课题
