摘要
采用反转录 聚合酶链反应(RT PCR)技术,从鸡胚、鹅胚中扩增得到肌肉生成抑制因子基因,分别克隆到pGEM TEasy载体中进行序列测定.结果表明,克隆得到的MSTN基因序列均由1128个核苷酸组成,MSTN基因序列同源性为94 6%,推导相应氨基酸序列的同源性达97 9%.
The myostatin gene of chicken and goose was amplified by reverse transcription-polymerase chain reaction(RT-PCR),then cloned into pGEM-T Easy vector and sequenced separately. The result showed that the complete gene either obtained from chicken or goose skeletal muscle consisted of (1 128) nucleotides, chicken myostatin cDNA sequence shared 94.6% homology with goose myostatin cDNA sequence,and the homology of myostatin amino acids sequence between them was 97.9%, which was higher than that of the cDNA sequence.
出处
《华南农业大学学报》
CAS
CSCD
北大核心
2004年第2期85-88,共4页
Journal of South China Agricultural University
