摘要
小鼠胸腺上皮细胞株 MTEC_1细胞与考的松耐受胸腺细胞(CRT)共同培养,在无任何外源刺激剂条件下,MTEC_1细胞可维持 CRT 存活,促进 CRT 增殖,双荧光抗体染色 FACS 分析证明,MTEC_1细胞选择性地支持 CD_4^+CD_8^-细胞增殖。在 Con A 活化条件下,MTEC_1细胞仍以促进 CD_4^+CD_8^-细胞增殖为主.MTEC_1细胞培养上清液(MTEC_1—SN)不能直接促进 CRT 增殖,但可维持其存活;在 Con A 活化条件下,则可促进 CRT 增殖。表型分析发现,MTEC_1—SN 对 CD_4^+CD_8^-和 CD_4^-CD_8^+细胞的存活和增殖无明显选择作用。说明 MTEC_1细胞对 CD_4^+CD_8^-细胞的选择作用是两种细胞直接作用的结果.
MTEC1 cell line,established in our laboratory,was identified as epithelial cells in nature and derived from ormal Balb/c mouse thymus medulla.To study the impact of MTEC1 cells on thedeveloping of mature thymocytes,we ocultured MTEC1 cells or/and MTECI cell culture supertants(MTEC1—SN)with cortision resistant thymocytesCRT).The results showed that,without anyadditional stimulators,MTEC1 cells could maintain the viability f RT cells,and slightly butsignificantly promote CRT cells to proliferate.MTEC1—SN alone could maintain the viability fCRT cells,but not promote CRT cells to proliferate.Added suboptical doses of Con A into medium,the number of the iable CRT cells and the extent of proliferaton of CRT cells were significantlyincreased when CRT cells was cocultured ith MTECI cells and/or MTEC1—SN.The phenotypes of viable CRT cells after cocultured with MTEC1 cells or TEC1—SN wereanalysed on flow cytometry(FACS).The viable CRT cells were seperated by density cut aftercocultured with MTECI cells or MTEC1—SN for 5 days,then stained with PE-anti-L3T4 and FITC-anti-Ly2 cAbs.The results showed that MTEC1 cells selectively promoted CD4^+CD8-thymocytesto proliferate,the ratio of CD4+CD8^-/CD4^-CD8^+ increased from 1.4 to 3.1.Under thBe conditionof MTEC1 cells cocultured with Con A activated RT ells,MTEC1 cells also mainly promoted CD4^+CD8^-thymocytes to proliferate.MTEC1-SN alone could maintain the iability of CD4^+CD8^-and CD4^-CD8^+thymocytes,andpromoted Con A activated CD4^+CD8^- and CD4^-CD8+thymocytes to proliferate,the propotion oftwo subsets was 34.6±3.5% and 49.7±6.1%.These studies emostrated that MTEC1 cell linepossess selective functions on the intrathymic T cell evelopment.
出处
《中国免疫学杂志》
CAS
CSCD
北大核心
1992年第1期25-30,共6页
Chinese Journal of Immunology
基金
美国洛氏中华医学基金(89-523)
国家自然科学基金资助课题
关键词
胸腺上皮细胞
细胞系
增殖
CRT
Thymic epithelial cell
cell line
Cortision resistant thymocyte
Proliferation
Selection
