摘要
目的 :为加快白头翁种苗生产速度及其生产的现代化。方法 :利用组织培养的方法 ,在添加不同种类植物激素处理组合的培养基上 ,对不同的外植体进行初代培养、丛生芽继代培养及其生根培养进行研究。结果 :适宜于白头翁的初代培养基 :MS + 6 BA 1.0～ 3 0mg·L- 1+NAA 0～ 0 .0 5mg·L- 1+蔗糖 3 0g·L- 1;增殖培养基 :MS + 6 BA 0 .2mg·L- 1+NAA 0 .0 2mg·L- 1+BR 0 .0 0 0 0 1mg·L- 1+蔗糖 3 0g·L- 1;生根培养基 :1/ 2MS +NAA 0 .4mg·L- 1+蔗糖 2 0g·L- 1。结论 :采用白头翁的茎尖和花蕾作为外植体 ,利用组织培养的方法能够实现种苗的工厂化快速繁殖。
Objective: To speed up seedling production of pasqueflower (Pulsatilla chinenses)and their modernization in pasqueflow er. Method: With tissue culture method, primary culture of different explants ,culture of cluster buds and their rooting culture were conducted on medium of treatment combinatio ns of adding different hormones. Result: The appropriate m edium for different culture stages were MS + 6-BA 1.0～3.0 mg·L -1 + NAA 0～0.05 mg·L -1 + Sucrose 30 g·L -1 in primary culture, MS + 6-BA 0.2 mg·L -1 +NAA 0.02 mg·L -1 + BR 0.000 01 mg·L -1 + Sucro se 30 g·L -1 in differentiation and subculture of cluster buds, 1/2 MS+ NAA 0.4 mg·L -1 + Sucrose 20 g·L -1 in rooting. Conclu sion: Applying stem tip and flower buds as explants , high frequency propagation of seedlings can be achieved with plant tissue culture in Pasqueflo wer.
出处
《中国中药杂志》
CAS
CSCD
北大核心
2004年第3期215-218,共4页
China Journal of Chinese Materia Medica
基金
安徽省教育厅资助项目 ( 2 0 0 3KJ0 6 6 )
