摘要
本文采用溶葡萄球菌素和溶菌酶溶菌,链霉素沉淀酶蛋白和肝素-琼脂糖CL-6B亲和层析法,从金葡球菌FDA 209P中提取DNA螺旋酶。当酶的A与B亚单位重组后,才显示出酶活性,使松弛的质粒DNA(pBR322)转为超螺旋结构。氧氟沙星,L-氧氟沙星、环丙沙星、诺氟沙星和萘啶酸抑制50％酶活性的浓度(IC_(50))分别为26、19、36、180和>400μg/ml。抑酶作用与抗菌活性(MIC)有一定相应关系。
DNA gyrase from Staphylococcus aureus FDA 209P was purified by bacteria lysis using lysostaphin lysozyme, precipitation with streptomycin and affinity chromatography on heparin Sepharose CL-6B.A or B subunits in the absence of the other subunit had no enzymatic activities which were detected by the conversion of relaxed pBR322 DNA to the supercoiled form.The 50% inhibitory concentrations(IC50s)of ofloxacin, L-ofloxacin, Ciprofloxacin, norfloxacin and nalidixic acid were 26, 19, 36, 180 and >400μg/ml respectively.The inhibitory effects of 5 quinolones on the enzyme were correlated with MICs.
出处
《中国抗生素杂志》
CAS
CSCD
北大核心
1992年第3期188-191,共4页
Chinese Journal of Antibiotics
基金
中国科学院自然科学基金资助项目
关键词
喹诺酮类
金葡球菌
DNA螺旋酶
Quinolones
Resistance
Staphylococcus aureus DNA gyrase
