摘要
研究了在乳化剂OP微乳液介质中钼 (Ⅵ ) 3′ ,5′ 二溴水杨基荧光酮 (3′ ,5′ DBSF) 蛋白质体系的相互作用和吸收光谱情况。在最佳条件下 ,牛血清蛋白质含量在 0～ 10mg/L范围内服从比尔定律 ;同时测得络合物与蛋白质的结合数n =93。乳化剂OP微乳液引入到蛋白质的测定中提高了体系的灵敏度。结果表明 :该法具有很高的灵敏度、选择性和稳定性 ,可直接用于人尿、奶粉和人血清样品的定量测定。
The interaction and absorption spectral behavior of 3′,5′-dibromosalicylfluorone (3′,5′-DBSF)-Mo(Ⅵ) and protein were studied in emulsion OP microemulsion medium. Under optimum condition, Beer's law is obeyed in the range of 0～10 mg/L of bovine serum albumin (BSA). Moreover, the binding number of the complex and BSA is 93. When the emulsion OP microemuslion medium was introduced into the protein solution, the sensitivity was enbanced for the determiantion of protein. The results indicate that the method possesses high sensitivity as well as high selectivity. It has been used to determine protein in human urine, milk powder and human serum with satisfactory results.
出处
《分析化学》
SCIE
EI
CAS
CSCD
北大核心
2004年第3期370-373,共4页
Chinese Journal of Analytical Chemistry
基金
山东省自然科学基金资助 (No .Y2 0 0 0B0 9)
