摘要
目的 :了解本地区血 (体 )液中临床感染细菌的分布情况。方法 :采用 Mini VITAL全自动荧光血培养仪和 VITEK32自动细菌鉴定仪对我院 1999年 1月～ 2 0 0 2年 12月临床送检的 836 2人份 16 395瓶血 (体 )液标本的培养结果作回顾性分析。结果 :16 395瓶临床血 (体 )液标本需氧和厌氧培养 ,3894瓶细菌培养阳性 ,总阳性率 2 3.8% (血液 2 3.2 % ,体液 2 8.6 % ) ,专性厌氧菌占除甲型副伤寒沙门菌外的其他感染菌的 3.7% ;共检出各种感染菌 4 1属 96种 2 36 8株 ,革兰阴性杆菌∶革兰阳性球菌∶酵母样真菌 =86 .0∶ 11.8∶ 0 .6 ;有 77.3%的兼性厌氧菌同时在需氧和厌氧环境都生长 ,10 .3%和 12 .4 %只在需氧或厌氧培养中生长。结论 :除传染病因素外 ,血 (体 )液中的感染菌群组成以革兰阳性球菌为主 ;同时做血 (体液 )标本的需氧和厌氧培养 ,可以提高细菌培养阳性率。
Objective: To study the floma distribution of clinical infection bacteria in blood and body fluid in Yuxi municipal.Methods:The retrospective investigation of bacteria isolated from 8 362 cases 16 395 bottles of clinical examples of blood and body fluid from Jan.1999 to Dec.2002 in our hospital by Mini VITAL Blood Culture Automated System and VITEK-32 Automicrobic System.Results:In aerobic and anaerobic culture of 16 395 bottles clinical samples of blood and body fluid,3 894 bottles(22.8%) showed bacteria positive results (the positive rate of blood was 23.2%,body fluid was 28.6%). In which 3.7% showed obligate anarobic positive of all isolated bacteria besides Salmonella paratyphi A.These isolates included 41 species and 96 types and 2 368 strains of bacteria.These ratio of Gram-negative bacillus and Gram-positive coccus and Saccharomyccs were 86.0∶11.8∶0.6.Among 4 144 specimens of both aerobic and anaerobic that yielded positive culture,a pure acrobic and anaerobic that yielded positive culture,a pure acrobic bateria were found in 10.3%,a pure anaerobic in 12.4%,both in 77.3%.Conclusion:The Gram-positive coccus was main isolated bacteria of clinical samples in blood and body fluid besides the factor of infectious diseases.Applied both of aerobic and anaerobic culture,the positive detection rate has been raised.
出处
《中国微生态学杂志》
CAS
CSCD
2003年第5期295-296,共2页
Chinese Journal of Microecology
关键词
细菌谱
临床细菌
自动培养
自动培养
Bacterium spectrum
Clinical bacteria
Anaerabic culture
