摘要
目的 探讨中性粒细胞活化在冠心病发生发展中的病理作用和意义。方法 利用化学发光法检测活性氧 ,膜片钳技术检测中性粒细胞脱颗粒 ,分光光度计比色法检测血浆和中性粒细胞内髓过氧化物酶 (MPO)活性。结果 不稳定性心绞痛组和稳定性心绞痛组中性粒细胞产生活性氧的峰值均显著高于对照组 ,分别为 343 2 1± 6 6 4 ,32 5 6 5± 6 5 1和 2 71 5 3± 5 6 2 (P <0 0 1)。不稳定性心绞痛组高钙刺激膜电容最大变化值显著低于稳定性心绞痛组和对照组 ,分别为 (3 6 2± 0 31)pF ,(6 94± 0 4 3) pF和 (6 87± 0 36 ) pF(P <0 0 1)。不稳定性心绞痛组血浆和中性粒细胞内MPO活性均降低 ,稳定性心绞痛组血浆MPO降低 ,但细胞内MPO活性与对照组差别无显著意义。结论 中性粒细胞氧化和抗氧化代谢失衡可能参与了冠心病的发生发展 ,尤其在不稳定性心绞痛发病过程中起到重要作用。
Objective To investigate the production of reactive oxygen species and anti-oxidative protein of neutrophils in patients with coronary heart disease, and to evaluate the role of neutrophil activation in the pathogenesis of coronary heart disease. Methods Reactive oxygen species production was measured by chemiluminescence method, the degranulation of neutrophils was detected by the whole cell patch-clamp capacitance technique, the plasma and intracellular levels of myeloperoxidase(MPO) were quantified by photometry.Results The production of superoxide anion in neutrophils was increased in patients with stable or unstable angina more significantly than control group (344.21±64.4,325.65±65.1 vs 271.53±56.2,P<0.01). The maximal increase of cell membrane capacitance under high-concentration Ca 2+ stimulation was lower in unstable angina group than stable angina group and the control[(3.62±0.31) pF vs (6.94±0.43) pF, (6.87±0.36) pF, P<0.01]. Both the plasma MPO concentration and the intracellular MPO activity was found decreased in patients with unstable angina, while in stable angina group only the plasma MPO concentration was decreased, there is no significant difference of MPO activity between intracellular and the control. Conclusion Neutrophils could probablely take active part in both the initiation and the progression stages of coronary heart disease through their free radical generation and antioxidant degranulation.
出处
《中华检验医学杂志》
CAS
CSCD
北大核心
2003年第7期407-410,共4页
Chinese Journal of Laboratory Medicine
