摘要
在摇瓶中研究生物素对红发夫酵母生长和虾青素积累的影响。结果表明生物素的添加能促进细胞生长,但对细胞内虾青素含量的提高不利。添加10μg/L生物素时,最大比生长速率达到0.110h^(-1);添加6μg/L生物素时,达到最大细胞干重9.95g/L,比没有添加生物素时的7.53g/L提高32％.添加2μg/L生物素时,达到最高虾青素产量和产率,分别为3825μg/L和53μg/(L·h),比没有添加生物素时的2944μg/L和41μg/(L·h)提高30％.没有添加生物素时,达到最高细胞虾青素含量39μg/g.利用添加2μg/L生物素的方法可以提高虾青素产量,这种方法为提高虾青素产量提供了一种简单廉价的方法.
The effects of biotin on cell growth and astaxanthin accumulation of Phaffia rhodozyma were investigated in flasks. The addition of biotin promoted cell growth but decreased the cell astaxanthin content. The highest specific growth rate of 0.110 h-1 was achieved at 10 μg/L biotin. The highest cell dry weight of 9. 95 g/L was achieved at 6 μg/L biotin, which increased by 32% compared with 7.53 g/L when there was no biotin. As for astaxanthin production, the highest yield of astaxanthin of 3 825 μg/L and productivity of 53 μg/(L · h) were reached at 2 μg/L biotin, which increased by 30% compared with 2 944 μg/L and 41 μg/(L · h) with the absence of biotin, while the highest astaxantnin content of 391μg/g was reached at 0 μg/L biotin. This method provides a simple and inexpensive means to increase astaxanthin production in industrial fermentations.
出处
《华南理工大学学报（自然科学版）》
EI
CAS
CSCD
北大核心
2003年第6期80-84,共5页
Journal of South China University of Technology(Natural Science Edition)
基金
广东省自然科学基金(990671)
华南现工大学自然科学基金(E5321277)
