摘要
目的评价苦参碱脂质体的体外抗乙型肝炎病毒(HBV)作用。方法通过苦参碱脂质体、苦参碱作用于体外培养的2.2.15细胞,观察和比较二者对2.2.15细胞分泌HBsAg、HBeAg的影响及药物的细胞毒性,初步评价苦参碱脂质体的抗HBV作用。结果苦参碱脂质体、苦参碱作用于2.2.15细胞11 d后,对细胞的半数毒性浓度(TC50)分别为7.29mg/ml和1.33rage/ml,对HBsAg和HBeAg抑制的半数有效浓度(IC50)分别为0.078 mg/ml、3.35mg/ml、<0.078mg/ml和>10mg/ml,苦参碱脂质体对HBsAg和HBeAg的治疗指数(TI)分别为93.46和2.17,高于苦参碱的治疗指数。结论苦参碱脂质体在体外细胞培养中对HBsAg和HBeAg的分泌有较好的抑制作用,可以提高苦参碱的抗HBV作用。
Objective In vitro study the anti-HBV effect of liposome-encapsuled matrine(Lip-M) .Methods The in vitro effect of Lip-M and matrine on the secretion of HBsAg and HBeAg in the 2.2.15 cell Line. The toxicity of Lip-M and matrine on the 2.2.15 cell line was also studied.Results The TC50 of Lip-M and matrine on the 2.2.15 cell line was 7.29 ng/ml and 1.33 mg/ml respectively. IC50 of Up-M and matrine on the inhibition of HBsAg and HBeAg expression were 0.078 mg/ml and 3.35 mg/ml respectively.The treatment index(TI) of the Lip-M and matrine was 93.46 and 2.17 respectively. Conclusion In vitro test of Lip-M demonstrated a stronger inhibitory action on the secretion of HBsAg and HBeAg in 2.2.15 cell line, and enhanced the effect of matrine.
出处
《热带医学杂志》
CAS
2003年第1期19-21,共3页
Journal of Tropical Medicine
基金
广东省自然科学基金资助项目(No.990353/B)
广东省中医药管理局资助项目(No.099517/A)
