摘要
目的 探讨细胞凋亡在脊髓损伤发病机理中的作用。方法 采用 Allen氏方法制作大鼠脊髓损伤模型 ,用 HE染色及原位末端标记法 ,对大鼠脊髓损伤后不同时间脊髓组织中凋亡细胞的数目进行观察和分析 ,并作正常对照。结果 损伤后灰质和白质均检测到凋亡细胞 ,细胞凋亡发生在损伤后 2 h～ 4周 ,以 3d时达到高峰 ,至 4周时渐趋于正常。结论 大鼠脊髓损伤后神经细胞存在死亡和凋亡两种方式 。
Objective To study the role of apoptosis in spinal cord injury.Methods 54 rats with spinal cord injury were made with Allen weight drop and spinal cord tissues were removed from 2h to 4w after injury,the other group of 6 rats was contral.The apoptosis was observed by HE staining and TUNEL at the different time after spinal cord injury.Results Apoptotic cells appeared from 2h to 4w,and arrived to peak at 3d after injury in both gray and white matter.Conclusion Both of necrosis and apoptosis occur in neuron cells after spinal cord in jury in rats.
出处
《福建医药杂志》
CAS
2002年第6期8-9,共2页
Fujian Medical Journal
基金
福建省自然科学基金资助 (基金编号 F0 110 0 0 4)
