摘要
目的 建立小鼠局灶性线栓永久性和暂时性脑缺血模型 .方法 雄性ICR及DDY小鼠各 6 0只 ,分别行永久性脑缺血和暂时性脑缺血 ,经颈总动脉插入 8 0尼龙纤维线段 ,将其头端送至左侧大脑中动脉起始部 ,激光多谱勒流量仪监测大脑中动脉血流量 ,术后 2 4h的脑切片经TTC染色计算梗死灶的大小 .结果 永久性脑缺血模型ICR小鼠成功率为 96 % ,DDY小鼠成功率为 70 % ;而暂时性脑缺血模型ICR小鼠成功率为5 7% ,DDY小鼠成功率为 83% .ICR小鼠和DDY小鼠的永久性脑梗死体积分别为 (16 0± 32 )mm3和 (138± 15 )mm3;暂时性脑梗死体积分别为 (14 5± 17)mm3和 (12 1±l8)mm3.结论 建立了小鼠种系选择性局灶性线栓脑缺血模型 ,可分别用来研究永久性和暂时性脑缺血的病理生理过程 .
AIM To set up animal models in mice with permanent or transient focal cerebral ischemia by intraluminal occlusion using monofilament.METHODS Sixty ICR and sixty DDY mice were divided into two groups separately to study permanent and transient cerebral ischemia.An 8 0 segment of monofilament was inserted into the proximal position of left middle cerebral artery through carotid artery. The regional cerebral blood flow (rCBF) in the area of left middle cerebral artery was monitored by laser Doppler. 24 hours after the surgery, brains were cut into thick slices and stained with TTC, and the infarct volume was calculated. RESULTS The success rates of animal models of permanent focal cerebral ischemia in ICR and DDR mice were 96% and 70% respectively, while those of transient focal cerebral ischemia were 57% and 83% respectively.The infarct volume of permanent focal cerebral ischemia in ICR and DDR mice were (160±32)mm 3 and (145±17 )mm 3 respectively, while those of transient focal cerebral ischemia were (l38±15)mm 3 and (121±18)mm 3 respectively. CONCLUSION The species selective animal models are set up in mice with focal cerebral ischemia by intraluminal occlusion using monofilament, which can be used to study the pathophysiology of permanent or transient cerebral ischemia in different mouse species.
出处
《第四军医大学学报》
北大核心
2002年第23期2133-2136,共4页
Journal of the Fourth Military Medical University
基金
国家自然科学基金资助项目 (39970 2 66)
2 0 0 0年度日中医学协会交流项目资助
