摘要
α-Alkenes(terminal alkenes)are important fuel and platform chemicals that are mainly produced from petroleum.Microbial synthesis might provide a sustainable approach forα-alkenes.In this work,we engineered the methylotrophic yeast Pichia pastoristo produce long-chain(C15:1,C17:1 and C17:2)α-alkenes via a decarboxylation of fatty acids.Combinatorial engineering,including enzyme selection,expression optimization and peroxisomal compartmentalization,enabled the production of 1.6 mg/L α-alkenes from sole methanol.This study represents the first case of α-alkene biosynthesis from methanol and also provides a reference for the construction of methanol microbial cell factories of other high-value chemicals.
基金
financially supported by National Key Research and Development Program of China(2021YFC2103500)
National Natural Science Foundation of China(21922812 and 22161142008)
DICP innovation Grant(DICP I201920 and DICP I202111)from Dalian Institute of Chemicals Physics,CAS.
