摘要
The 3-Hydroxypropionic acid(3-HP)pathway is one of the six known natural carbon fixation pathways,in which the carbon species used is bicarbonate.It has been considered to be the most suitable pathway for aerobic CO_(2)fixation among the six natural carbon fixation pathways.Mesaconate is a high value-added derivative in the 3-HP pathway and can be used as a co-monomer to produce fire-retardant materials and hydrogels.In this study,we use mesaconate as a reporting compound to evaluate the construction and optimization of the sub-part of the 3-HP pathway in Saccharomyces cerevisiae.Combined with fine-tuning of the malonyl-CoA reductase(MCR-C and MCR-N)expression level and optimization of 3-Hydroxypropionyl-CoA synthase,the 3-HP sub-pathway was optimized using glucose or ethanol as the substrate,with the productions of mesaconate reaching 90.78 and 61.2 mg/L,respectively.
基金
supported by the National Key Research and Development Program of China[Grant Number 2018YFA0900100]
the China Postdoctoral Science Foundation[Grant Number 2021M690321]
the Beijing Advanced Innovation Center for Soft Matter Science and Engineering,Beijing University of Chemical Technology.
