摘要
Increasing the sucrose content of sugarcane,a major sugar crop,is a key breeding objective.However,the complex genetic background of sugarcane affects development of sugarcane hybrids.In this study,we sequenced 292 sugarcane germplasm accessions and identified 2,542,965 single nucleotide polymorphisms(SNPs) and insertions/deletions(InDels).We performed a genome-wide association study(GWAS) for two important sugarcane traits:sucrose content and stem diameter.Both traits followed a normal distribution and showed typical characteristics of quantitative traits.Population structure analysis revealed four subpopulations with an average genetic distance of 0.236.GWAS of the sucrose content detected 27 SNPs.After annotating genes at or near significant loci,17 candidate genes were screened.For stem diameter,GWAS revealed 19 SNPs,from which 9 candidate genes were identified.These results improve our understanding of genetic mechanisms affecting sucrose content in sugarcane,and identify important genetic resources to accelerate breeding of new sugarcane varieties with high sucrose content.
基金
supported by the National Natural Science Foundation of China (32060505, 31660418)
the Project of National Key Laboratory for Tropical Crop Breeding, China (NKLTCB202318)
the Yunnan Seed Laboratory, China (202205AR070001-13)
the Technology Innovation Talents in Yunnan Province, China (202305AD160041)
the National Sugar Industry Technology System, China (CARS-170101)
the High-performance Computing Platform of YaZhou Bay Science and Technology City Advanced Computation Center, China。
