摘要
Objective:Previous antibiotic therapy is acknowledged to potentially reduce the efficacy of single-agent immune checkpoint inhibitors.Nevertheless,the impact of antibiotics on the results for patients undergoing chemoimmunotherapy remains un-clear.This research investigated the influence of antibiotic treatment on the effectiveness of chemoimmunotherapy in ad-vanced non-small cell lung cancer(NSCLC).Methods:We recorded the characteristics of patients with advanced NSCLC and assessed potential associations between the use of antibiotics and the efficacy of chemoimmunotherapy.A mouse model using Lewis lung carcinoma(LLC)cell lines was developed to assess the effects of antibiotics on the gut microbiome and metabolites.Fecal samples were analyzed using 16S rRNA gene sequencing and ultra-high-performance liquid chromatography–mass spectrometry(UHPLC-MS)methods.Mouse fecal and serum samples and 16 human stool samples were used to validate the identified differentially metabolites.Deoxy-cholic acid(DCA)was further applied to a LLC mouse model.Results:This study included 387 NSCLC patients,among whom 86 patients had used antibiotics within the 30 days before the first cycle of chemoimmunotherapy(ATB group),and 301 patients had not used antibiotics(non-ATB group).Notable discrep-ancies were observed in overall survival and progression-free survival between the two groups,with overall survival recorded at 18.4 months versus 32.0 months,and progression-free survival at 7.6 months versus 13.0 months,in the ATB and non-ATB groups respectively.At the phylum level,the relative abundances of Proteobacteria,Cyanobacteria,and Deinococcus were in-creased in the ATB mice,while Firmicutes,Bacteroidetes,and Verrucomicrobia were decreased.We detected significant differ-ences in DCA levels in the fecal and serum samples from mice as well as in the fecal sample from humans between the ATB and non-ATB groups.The respective proportions of CD4+and CD8+cells were greater in the non-ATB group than in the ATB group,whereas the proportion of Ki67-positive cells was greater in the ATB group.DCA was applied to LLC mice,and DCA along with chemoimmunotherapy effectively inhibited tumor growth in a LLC mouse model.The expression of programmed cell death ligand 1 increased in the DCA group.Conclusions:Antibiotic exposure is associated with decreased efficacy of chemoimmunotherapy in patients with NSCLC via dysregulation of the gut microbiome and DCA metabolism.
基金
supported by the National Natural Science Founda-tion of China(grant No.82370085 to C.C.)
Wujieping Medical Foun-dation Clinical Research Special Fund(grant No.320.6750.2025–6-88 to Y.L.)
Wenzhou Municipal Science and Technology Bu-reau(grant No.Y2020001 to H.X.).
