摘要
Citrus reticulata cv.Chachiensis(CR)pulp is often directly landfilled during agricultural production,causing environmental pollution and the loss of thermosensitive bioactive substances.Non-thermal processing shows advantages in efficiently extracting such thermosensitive compounds and preserving their bioactivities.In this study,the high-activity fraction CU-N was prepared through a directional strategy combining pulsed electric field(PEF)-ultrasonic non-thermal extraction,ethanol polarity fractionation,and NKA-9 macroporous resin purifi-cation.CU-N significantly enhanced the antioxidant capacity of Caenorhabditis elegans(C.elegans)by reducing MDA(0.37±0.05 nmol/mgprot)and increasing T-SOD(68.16±1.28 U/mgprot)and CAT(7.75±0.16 U/mgprot)levels.The main components identified in CU-N were neohesperidin,isosakuranetin-7-O-rutinoside,naringenin 4′-glucoside 7-rutinoside,narirutin,and kaempferol 3-O-β-D-glucopyranosyl-7-O-α-L-rhamnopyr-anoside.Mechanistically,CU-N was predicted to enhance antioxidant capacity through regulation of autophagy,longevity-regulating signaling pathway,and the one carbon pool by folate metabolism pathway based on transcriptomics and metabolomics.Furthermore,the key small-molecule components within CU-N(CRF)were predicted to target CYP1B1,TNF,EGFR,and PTGS2,suggesting their potential role in intervening against dia-betic nephropathy(DN).These results clarified the antioxidant mechanism of CU-N and provided a scientific basis for the targeted preparation of high-activity functional components from CR and the development of CRderived functional foods with antioxidant and DN-intervening activities.
基金
supported by the Key R&D Program Projects in Guangdong,China(2024B0202010001 and 2023B0202040002)
the Science and Technology Planning Project in Guangdong,China(SDZX2023036)
the‘Open Competition’Science&Technology Research Project of National Excellent Engineer Innovation Research Institute for Advanced Manufacturing Industry in Guangdong Hong Kong Macao Greater Bay Area(Foshan),China(JBGS2024003).
