摘要
Microbial secondary metabolites are precious bioactive compounds used as natural food preservatives against foodborne pathogens.Biosynthetic gene clusters(BGCs),responsible for the biosynthesis of secondary metabolite,provide crucial information for the efficient discovery of novel products.Lactobacillaceae,intimately associated with the human daily diet throughout history,is an important and non-negligible secondary metabolite resource.However,the global secondary metabolic potential in the Lactobacillaceae family was still largely unclear.In this study,we performed a large-scale genome mining for biosynthetic potential of secondary metabolites across 515 Lactobacillaceae members,involving 10,327 genomes from multiple ecosystems.A total of 18,363 BGCs were determined from 85.44%of the Lactobacillaceae genome,suggesting the ubiquitous biosynthetic potential of secondary metabolite.Based on BiG-SCAPE analysis,these BGCs were clustered into 1377 gene cluster families(GCFs),and these GCFs had high interspecies specificity(72.98%)and intraspecies heterogeneity(94.74%).The human body,animal body,and fermented food together harbored diverse Lactobacillaceae taxa(153 species)and biosynthetic potentials(414 GCFs)with 80.23%habitat specificity.Novelty analysis indicated 1057 GCFs(76.76%)were distantly related to the reference dataset.Biological activity analysis suggested that post-translationally modified peptide(RiPP)contained the most number of novel GCFs with high probabilities(≥80%)of antibacterial activity(271 GCFs).This study provided a new blueprint of the biosynthetic potential of secondary metabolite in Lactobacillaceae and novel insights into the potential application of Lactobacillaceae to combat foodborne pathogens.
基金
supported by the National Natural Science Foundation of China(32172175)
the Priority Academic Program Development of Jiangsu Higher Education Institutions(No.111-2-06)
the Jiangsu Basic Research Center for Synthetic Biology Grant(No.BK20233003)
Postdoctoral Fellowship Program of CPSF(GZC20240728)
the Jiangsu Funding Program for Excellent Postdoctoral Talent(2024ZB271)
Project of Fund for Stable Support to Agricultural Sci-Tech Renovation(xjnkywdzc-2024001).
