摘要
Persistent low-grade systemic inflammation is intrinsically linked to aging and degenerative diseases,with gut microbiota dysbiosis serving as a key intermediary.Animal-derived proteins are naturally abundant in terminal sialylated glycans,which aid to modulate gut microbiota composition and attenuate inflammatory responses.In contrast,plant-based proteins such as soybean protein isolate(SPI)primarily contain high-mannose N-glycans and lack sialylation moieties.To address this nutritional gap,this study developed sialoglycan-enriched SPI by incorporating casein glycomacropeptide(CGMP),hydrolyzed ovomucin peptides(Eovm),and 3′-sialyllactose(3′-SL).Using a D-galactose-induced aging model in C57BL/6J mice,we evaluated the effects of these modified SPIs on physiological parameters,gut microbiota composition,oxidative stress markers,and inflammatory cytokines.Results showed that sialoglycan-enriched SPI significantly improved body weight gain,reduced blood glucose levels,enhanced SOD and CAT activities,and decreased MDA levels.Moreover,it suppressed the expression of TNF-α,IL-1β,and IL-6.Microbiota analysis revealed increased diversity,enrichment of beneficial genera such as Lactobacillus and Bifidobacterium,and elevated production of short-chain fatty acids(SCFAs).Collectively,these findings suggest that sialoglycan-enriched SPI effectively mitigates D-galactose-induced oxidative stress and inflammation,offering promising potential for the development of anti-aging functional foods based on plant proteins.
基金
supported by the National Key Research and Development Program of China(2023YFA0914303).
