摘要
Zein-phloretin-stevioside ternary complex(Zein-PT-STE)were prepared using reverse solvent precipitation to deliver PT and enhance its in vitro activity.The complex exhibited an average particle size of 349.9 nm,a polydispersity index(PDI)of 0.160,and an entrapment efficiency of 88.09±1.04%.Structural characterization was performed using spectroscopic and thermal analyses,while the assembly mechanisms were investigated through molecular docking and molecular dynamics simulations.Ultraviolet spectroscopy and FT-IR revealed strong binding between zein and both PT and STE,enhancing the hydrophilicity and changing its secondary structure of the protein.XRD and DSC indicated that PT was effectively encapsulated in the Zein-PT-STE complex with amorphous state,improving the water solubility of PT.Molecular docking(binding energy:-10.847 kcal/mol)and molecular dynamics simulations further demonstrated the complex’s significant binding stability.In vitro simulated digestion experiments revealed that Zein-PT-STE promotes the intestinal release of phloretin.Additionally,the complex exhibited synergistically enhanced antioxidant capacity(DPPH(1,1-diphenyl-2-picrylhydrazyl))and ABTS(2,2′-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid))andα-glucosidase inhibitory activity.The result indicate that Zein-PT-STE complex formation and structural stability were driven by hydrogen bonding and hydrophobic forces.This study elucidates the key mechanisms governing the formation and stability of the Zein-PT-STE ternary complex,offering enhanced stability and functionality for diverse PT delivery applications.
基金
funding from the Livelihood Plan Project by the Department of Science and Technology of Liaoning Province(2021JH2/10300069,2019-ZD-0845)
the Department of Education of Liaoning Province(LJ212410163061).
