摘要
The strong infectivity and widespread resistance of methicillin-resistant Staphylococcus aureus(MRSA)urgently require the development of new antibacterial agent.Cedrol is a natural terpenoid with antimicrobial properties.To our knowledge,this is the first study to explore the antibacterial mechanism of cedrol against MRSA using transcriptomic and molecular docking methods.Cedrol exhibited good antibacterial activity against MRSA,with a minimum inhibitory concentration of 0.32 mg/mL and a minimum bactericidal concentration of 0.48 mg/mL.Cedrol-treated MRSA exhibited growth inhibition,significant changes in cell morphology,loss of membrane integrity,increased membrane permeability,and leakage of cell contents.Moreover,transcriptomics confirmed that cedrol caused damage to the MRSA cell wall and membrane,dysregulation of the membrane transport system,abnormalities in ribosome and protein synthesis,global disruption of cellular metabolism,and diminished MRSA infectivity.The molecular docking results indicated that GlnA and RplT in MRSA may be potential targets of cedrol.Hemolysis and cytotoxicity tests showed that the hemolysis rate was<5%and the cell survival rate was>95%,indicating that cedrol had good biological safety.Finally,cedrol effectively inhibited the growth of MRSA in the chicken soup.These findings provide a theoretical basis for the development of cedrol as a novel natural food antibacterial agent.
基金
financially supported by Hubei Provincial Natural Science Foundation of China(2023AFB927)
Key Research and Devel-opment Program of Hubei Province(2023BBB010)
Hubei Natural Sci-ence Fund Project(2025AFC028)
Major Science and Technology Innovation Plan Project of Jingmen City(2024ZDYF15)
Partially Supported Open Project Funding of the Key Laboratory of Fermentation Engineering(Ministry of Education)(202409FE13).
