摘要
Spontaneous fermentation of young ginger pickles(YGP)involves numerous indigenous microbes from raw materials and the environments,while inoculated fermentation is an ideal strategy for standardizing YGP production and improving quality.This study investigated the effects of exogenous inoculation with Lactiplantibacillus plantarum ATCC 8014 on the quality characteristics and metabolic profile of YGP.It particularly explored the response of indigenous microbes,focusing on their interactions and metabolic characteristics in relation to exogenously inoculated microbes.The results indicated that L.plantarum inoculation significantly increased organic acid levels,while decreasing nitrite content during YGP fermentation.In addition,L.plantarum-inoculated YGP exhibited increased Lactiplantibacillus abundance and significant alterations in color and texture characteristics when compared to naturally fermented YGP.Beta diversity analysis revealed improved similarity of fermented microbial community structure,while co-occurrence network analysis showed that L.plantarum inoculation increased interaction complexity,the positive correlation ratio,and the number of keystone taxa,particularly strengthening the symbiotic relationships among LAB.Furthermore,a total of 27 volatile and 29 non-volatile differential metabolites were identified between inoculated and uninoculated YGP.These differential metabolites were linked to the degradation of valine,leucine,and isoleucine,as well as pyruvate metabolism and glycolysis/gluconeogenesis pathways.Correlation network analysis suggested that the differential LAB significantly enhanced the quality attributes and metabolic profiles of YGP,likely due to the driving effects of L.plantarum on the symbiotic relationships among indigenous LAB.Overall,these findings provided valuable insights into the management of YGP fermentation and the standardization of its quality.
基金
supported by the’Nan Hai Xin Xing’Science and Technology Innovation Talent Platform Project Funding of Hainan Province(NHXXRCXM202312)
Hainan University Scientific Research Start-up Fund Project(Grant No.XJ2300006565).
