摘要
As a promising resource,animal bones have been widely utilized in functional foods designed to promote bone health.However,few studies were conducted to assess the efficacy of animal bones,particularly regarding its potential in modulating disorders of gut microbiota.In this study,ovariectomized osteoporotic mice were intervened with low,medium,and high doses of yak bone meal(YB),focusing on its anti-osteoporosis properties and regulatory impact on gut microbiota in vivo.The results showed that YB treatment significantly increased indicators of femoral trabecular bone,including trabecular number(Tb.N);trabecular thick(Tb.Th);percent trabecular area(Tb.Ar);and cortical thickness,while reducing osteoclast number(OC)and trabecular separation(Tb.Sp).Notably,the high-dose YB group(0.95141 g/kg⋅BW)exhibited effects on bone microstructure similar to those observed with the positive calcium group(1.0704 g/kg⋅BW),indicating strong anti-osteoporotic effect.Furthermore,YB intervention enhanced the density and diameter of muscle fibers.These alleviated symptoms of skeletal muscle atrophy.Additionally,YB improved gut microbiota dysbiosis induced by osteoporosis,evidenced by raised Firmicutes/Bacteroidetes ratio and increased abundance of Lactobacillus,which was demonstrated beneficial for bone health.Correlation analysis showed that both Firmicutes and Lactobacillus were significantly positively correlated with Tb.N,Tb.Th,Tb.Ar,cortical thickness index,thymus index,gastrocnemius index and quadriceps femoris index,and significantly negatively correlated with Tb.Sp,OC,interfascicular distance,and intermuscle fiber distance.These findings suggest that YB may serve as valuable supplement for alleviating osteoporosis and simultaneously regulating gut microbiota disorders in vivo.
基金
supported by the Fundamental Research Funds for the Central Universities(31920230159,31920240106)
National Natural Science Foundation of China(32260617).
