摘要
This study screened for the inhibitory peptides from the collagen of barracuda fish skin then determined its inhibitory effect on hyperlipidemia.The results showed that the collagen was type I,mainly containingα1,α2,andβchains.Scanning electron microscopy revealed the fiber structure,sheet structure,multilayer aggregation structure,which was loose and porous.For an optimal enzymatic digestion process the pH was 10,the dosage of alkaline protease was 3000 U/g,and the temperature 50℃,with a degree of hydrolysis of 26.03%.The peptides of less than 1 KDa were selected by protein ultrafiltration and LC-MS identification revealed 220 peptides.The results indicated that GAPGFPGPR can be the best inhibitory peptide based on its bioactivity,water solubility,toxicity,ADMET and molecular docking technology.In vitro experiment showed that the GAPGFPGPR had significant inhibitory effects on the cholesterol ester transfer protein.The zebrafish experiment further verified that the GAPGFPGPR significantly reduced the total cholesterol,triacylglycerol,low-density lipoprotein cholesterol levels and increased the high-density lipoprotein cholesterol levels in the hyperlipidemia model.Therefore,the study has demonstrated the potented for developing health care products or drugs to improve hyperlipidemia.
基金
supported by the Open Fund of Institute of Ocean Research,Bohai University(BDHYYJY2024003).
