摘要
The aim of this study was to investigate the structure and stability of silver carp skin collagen peptide-calcium chelate(SSCPs-Ca)and evaluate its effectiveness in promoting calcium absorption.SSCPs were prepared by twostep enzymatic hydrolysis and chelated with calcium to form SSCPs-Ca.The structure of SSCPs-Ca was characterized by ultra-violet spectroscopy,fluorescence spectroscopy,particle size distribution,scanning electron microscopy and atomic force microscopy.Then explored the effects of temperature,lactose,sodium chloride and phosphate on its stability.The calcium uptake of SSCPs-Ca was evaluated using Caco-2 cell and zebrafish osteoporosis models.Compared to SSCPs,SSCPs-Ca exhibited notable improvements in particle size and adopted a stable,osteocalcin-like crystal structure.SSCPs-Ca exhibited enhanced electrostatic repulsion and thermal stability.SSCPs-Ca significantly enhanced calcium transport and absorption at the cellular level,outperforming CaCl2.Unlike traditional supplements,its efficiency remained unaffected by dietary components such as lactose and NaCl.SSCPs-Ca operated independently of Vitamin D for calcium transport,emphasizing its innovative mechanism.SSCPs-Ca at a concentration of 125μg/mL demonstrated superior efficacy in mitigating osteoporosis in zebrafish models,achieving comparable results to etidronate disodium at a concentration of 300μg/mL.These findings established SSCPs-Ca as a groundbreaking calcium supplement with the potential to significantly improve bone health.By addressing calcium deficiency and associated risks with enhanced bioavailability and stability,SSCPs-Ca offered a promising alternative to traditional calcium supplements,paving the way for innovative strategies in managing calcium-related health challenges.
基金
supported by China Agriculture Research System(Program No.CARS-45-27).
