摘要
Glycerylphosphorylcholine(GPC)is a bioactive compound with potential health benefits,including cognitive support and cellular metabolism enhancement.In this study,GPC-enriched defatted egg yolk powder was prepared using an innovative combination of dual-enzyme hydrolysis and supercritical CO_(2) extraction.Optimized conditions achieved a high conversion rate of phosphatidylcholine(PC)(89.9%).Key parameters included a 2:1 enzyme ratio(phospholipase A1 to lysophospholipase),161 U/mL enzyme concentration,3-h reaction time,55℃ temperature,and a 1:2 yolk-to-water ratio.Furthermore,under the same conditions,the content of GPC reached 0.374 g per 10 g of egg yolk powder.Supercritical CO_(2) extraction increased the protein content and GPC content of the egg yolk powder from 29.9%to 68.68%and 3.74%–7.75%while reducing the lipid content and cholesterol content from 50.1%to 6.75%and 4.21%–0.16%.Accelerated storage tests revealed that as the storage time increased,the changes in the particle size and secondary oxidation products of EYP161-SYE were less pronounced,which correlated with improved solubility and storage stability.These findings highlight the potential of this approach for producing high-value,stable GPC-enriched egg yolk powder for applications in food and nutraceuticals.
基金
support from the National Key Research and Development Program of China(2022YFD2101004)
National Natural Science Foundation of China(No.32272313).
