摘要
Cardiomyopathies are often characterized by significant fibrotic remodelling of the heart,marked by an abnormal accumulation of collagen type I.Label free Raman spectroscopy,a non-invasive diagnostic technique,holds promise for monitoring biochemical changes throughout the initiation and progression of different diseases,including cardiomyopathies.This study demonstrates the effectiveness of 70%glycerol as a hyperosmotic immersion liquid for in-depth controlling the optical properties of ex vivo myocardium tissue during deep-UV Raman spectroscopy with 244 nm excitation.The results revealed a considerable enhancement in the intensities of Raman peak,particularly the amide I region after glycerol treatment.This occurred across all depths(0−120µm)and glycerol treatment durations(30 and 60 min).A noticeable enhancement of the Raman peak at 1647 cm^(−1) was also observed that is attributable to structural transformations of the collagen due to the dehydration induced by glycerol.This finding suggest that deep-UV Raman can be employed as a specific probe of the collagen environment.As the amide I region reflects structural changes in collagen type I,these findings propose the potential of deep-UV Raman spectroscopy in combination with glycerol as optical clearing agent for monitoring collagen modifications.
基金
the scholarship of the Stipendium Hungaricum Scholarship Programme,and supported by the VEKOP(2.3.2-16-2016-00011 and 2.3.2-16-2016-00002)grants
which are co-financed by the European Union and European Social Fund.This work was supported by the NEURAM(712821)
VISGEN(734862)H2020 projects the European Commission and by project no.TKP2021-NVA-04 provided by the Ministry of Innovation and Technology of Hungary from the National Research
Development and Innovation Fund(NKFIH),financed under the TKP2021-NVA funding scheme
VVT was supported by the Russian Science Foundation grant#23-14-00287.
