摘要
Depuration and anhydrous living-preservation are essential steps in the cold chain process for bivalves.However,the mechanisms by which these steps affect the quality of Pacific oysters(Crassostrea gigas)remain inadequately understood.In this study,the metabolic changes in Crassostrea gigas during these processes were investigated.Ultra-high-performance liquid chromatography-quadrupole-Orbitrap mass spectrometry(UHPLC-QE-Orbitrap/MS)was utilized,and multivariate statistical analysis was performed to extract and identify differential metabolites.A comprehensive analysis of the associated metabolic pathways was subsequently conducted,leading to the identification of differential metabolites,including amino acids,organic acids and fatty acids were identified.Among these,10 key differential metabolites,primarily comprising tyrosine,glutamic acid,glutamine,citrate,succinic acid,arginine,malic acid,L-isoleucine,L-valine and fumaric acid were highlighted.Furthermore,alanine,aspartate and glutamate metabolism,valine,leucine and isoleucine biosynthesis,phenylalanine,tyrosine and tryptophan biosynthesis,tricarboxylic acid(TCA)cycle,nitrogen metabolism and pyruvate metabolism were delineated.These findings reveal the key metabolites and metabolic pathways responsible for quality changes in Pacific oysters during depuration and anhydrous living-preservation.This study provides a theoretical basis for the quality control of Pacific oysters in cold chain circulation,which will help promote the development of the Pacific oyster industry chain.
基金
supported by the Tianchi Talent Introduction Plan Project of China(2224ZZQRCXM).
